Abstract
The presence of polyphenol oxidase enzyme (PFO) in black and white mulberry varieties grown in Iğdır province were determined. In the study, no polyphenol oxidase enzyme was detected in the white mulberry varietie, therefore the study was conducted only with black mulberry varieties. The mulberry samples were collected from Iğdır Center, Melekli and Karakoyunlu districts during June and July, both in 2015 and 2016, when the fruits were fully ripe. Mulberries were stored at -20 °C, and the crude enzyme extract was stabilized with pH 5.0 sodium-acetate buffer containing 1% Polyethylene glycol (PEG). To weigh with a precision scale, 20 grams of frozen mulberries were taken into mortars. After the mulberries were thoroughly crushed in these mortars, 40 ml of crude enzyme extract preparation solution was added to each variety and mixed homogeneously. These mixtures were filtered through a cheesecloth and rotated at 4 °C, 10,000 rpm for 30 minutes. The obtained supernatants were used for enzyme solubilization, and the enzyme activity of the solution was determined spectrophotometrically. The PFO content depended on the genotype. There was a significant difference among the tested mulberry genotypes.
References
- Bellini, E., Giordani, E., Roger, J.P. (2000). The mulberry for fruit. Informatore Agrario, 56(7), 89-93.
- Beşel, E., (2003). Use of Triton X-114 Aqueous Two Phase System for Recovery of Mushroom (Agaricus Bisporus) Polyphenoloxidase. Yüksek Lisans Tezi. ODTÜ, Ankara.
- Datta, R.K., 2002. Mulberry cultivation and utilization in India. Mulberry for Animal Production. FAO Animal Production and Health Paper, 147, 45-62.
- Demirel, F., Eren, B., Yilmaz, A., Türkoğlu, A., Haliloğlu, K., et al. (2023a). Prediction of grain yield in wheat by CHAID and MARS algorithms analyses. Agronomy, 13(6), 1438.
- Demirel, F., Uğur, R., Popescu, G.C., Demirel, S., Popescu, M. (2023b). Usage of machine learning algorithms for establishing an effective protocol for the ın vitro micropropagation ability of black chokeberry (Aronia melanocarpa (Michx.) Elliott). Horticulturae, 9(10), 1112.
- Erdoğan, Ü., Pırlak, L. (2005). Ükemizde dut (Morus spp.) üretimi ve değerlendirilmesi. Alatarım, 4(2), 38-43.
- Eren, B., Türkoğlu, A., Haliloğlu, K., Demirel, F., Nowosad, K., et al. (2023). Investigation of the influence of polyamines on mature embryo culture and DNA methylation of wheat (Triticum aestivum L.) using the machine learning algorithm method. Plants, 12(18), 3261.
- Freeman, W.H., 1978. Temparate-Zone Pomology. W.H. Freeman and Company, San Francisco. 428. Grieve, M., 2002. Mulberry Common. Online aveable: http://botanical.com/botanical/mgmh/m/mul.com62.html (01.05.2023).
- Huo, Y. (2002). Mulberry cultivation and utilization in China. Mulberry for animal production, FAO. Animal Production and Health Paper, 147, 11-44.
- Kovalchuk, I.Y., Mukhitdinova, Z., Turdiyev, T., Madiyeva, G., Akin, M., Eyduran, E., Reed, B.M. (2017). Modeling some mineral nutrient requirements for micropropagated wild apricot shoot cultures. Plant Cell, Tissue and Organ Culture, 129(2), 325-335.
- Machii, H., Koyama, A., Yamanouchi, H., Matsumoto K., Kobayashi, S., Katagiri, K. (2001). A list of morphological and agronomical traits of mulberry genetic resources. Misc. Publ. Natl. Inst. Seric. Entomol. Sci., 29, 1-307.
- Mahanta, P. K., Boruah, S.K., Boruah, H.K., Kalıta, N.J., 1993. Changes of poliphenol oxidase and peroxidase activites and pigment composition of some manufactured black teas (Camellia sinensis L.). Journal of Agriculture and Food Chemistry, 41, 272-276.
- Martin, G., Reyes, F., Hernandez, I., Milera, M. (2002). Agronomic studies with mulberry in Cuba. Mulberry for Animal Production, FAO Animal Production and Health Paper, 147, 103-114.
- MEB, 2013. http://megep.meb.gov.tr (09.04.2023).
- Rapeanu, G., Loey, A.V., Smouth, C., Hendrıckx, M. (2006). Biochemical characterization and process stability of poliphenoloxidase extacted from victoria grape (Vitis vinifera spp. sativa). Food Chemistry, 94, 253-261.
- Özbek, S. (1977). Genel Meyvecilik. Çukurova Üniversitesi Ziraat Fak., 111(6), Adana. 386.
- TUİK, 2014. Gıda, Tarım ve Hayvancılık Bakanlığı. https://www.tuik.gov.tr/ (12.05.2023).
- Türkoğlu, A., Bolouri, P., Haliloğlu, K., Eren, B., Demirel, F., et al. (2023a). Modeling callus induction and regeneration in hypocotyl explant of fodder pea (Pisum sativum var. arvense L.) using machine learning algorithm method. Agronomy, 13(11), 2835.
- Türkoğlu, A., Haliloğlu, K., Demirel, F., Aydin, M., Çiçek, S., et al. (2023b). Machine learning analysis of the ımpact of silver nitrate and silver nanoparticles on wheat (Triticum aestivum L.): Callus induction, plant regeneration, and DNA methylation. Plants, 12(24), 4151.
- Ünal, M. Ü. (2007). Properties of polyphenol oxidase from anamur banana (Musa cavendishii). Food Chemistry, 100(3), 909-913.
- Zheng, T., Tan, Y., Huang, G., Fan, H., Ma, B. (1988). Mulberry Cultivation. FAO Agriculturae Services Bulletin, 73/1, Rome, p127.
Abstract
Iğdır koşullarında yapılmış olan çalışmada siyah ve beyaz dut çeşitlerinin polifenol oksidaz enzim (PFO) varlığı tayin edilmiştir. Araştırma sonuçlarına göre ise beyaz dut çeşitlerinde polifenol oksidaz enzimine rastlanılmamış, bu nedenle sadece siyah dut çeşitleri ile çalışmaya devam edilmiştir. Çalışmada kullanılacak örnekler Iğdır Merkez, Melekli ve Karakoyunlu ilçelerinden 2015 ve 2016 yıllarında haziran ve temmuz aylarında meyveler tam olgunlaştıkları dönemde toplanmış ve laboratuar koşullarına getirilmiştir. Laboratuar koşullarına getirilen dutlar -20 °C’de dondurulmuş, % 1 Polietilen glikol (PEG) içeren pH’ı 5,0 sodyum-asetat tamponu ile ham enzim özütü hazırlama çözeltisi sağlanmıştır. Hassas terazi ile tartım yapmak için, dondurulmuş dutlardan 20’şer gram havanlara alınmıştır. Dutlar bu havanlarda iyice dövüldükten sonra her bir dut çeşidine 40 ml ham enzim özütü hazırlama çözeltisinden eklenmiş ve homojen bir şekilde karıştırılmıştır. Bu karışımlar bir tülbent yardımı ile süzülmüş ve 4 °C’de, 10.000 rpm’de 30 dakika santrifüj edilmiştir. Elde edilen süpernatanlar enzim çözeltisi olarak kullanılmış, her bir çözelti de ayrı ayrı enzim aktivitesi spektrofotometrik olarak tayin edilmiştir. PFO içeriği dut genotiplerine bağlı olarak istatistiksel olarak önemli farklılıklar gösterdi.
References
- Bellini, E., Giordani, E., Roger, J.P. (2000). The mulberry for fruit. Informatore Agrario, 56(7), 89-93.
- Beşel, E., (2003). Use of Triton X-114 Aqueous Two Phase System for Recovery of Mushroom (Agaricus Bisporus) Polyphenoloxidase. Yüksek Lisans Tezi. ODTÜ, Ankara.
- Datta, R.K., 2002. Mulberry cultivation and utilization in India. Mulberry for Animal Production. FAO Animal Production and Health Paper, 147, 45-62.
- Demirel, F., Eren, B., Yilmaz, A., Türkoğlu, A., Haliloğlu, K., et al. (2023a). Prediction of grain yield in wheat by CHAID and MARS algorithms analyses. Agronomy, 13(6), 1438.
- Demirel, F., Uğur, R., Popescu, G.C., Demirel, S., Popescu, M. (2023b). Usage of machine learning algorithms for establishing an effective protocol for the ın vitro micropropagation ability of black chokeberry (Aronia melanocarpa (Michx.) Elliott). Horticulturae, 9(10), 1112.
- Erdoğan, Ü., Pırlak, L. (2005). Ükemizde dut (Morus spp.) üretimi ve değerlendirilmesi. Alatarım, 4(2), 38-43.
- Eren, B., Türkoğlu, A., Haliloğlu, K., Demirel, F., Nowosad, K., et al. (2023). Investigation of the influence of polyamines on mature embryo culture and DNA methylation of wheat (Triticum aestivum L.) using the machine learning algorithm method. Plants, 12(18), 3261.
- Freeman, W.H., 1978. Temparate-Zone Pomology. W.H. Freeman and Company, San Francisco. 428. Grieve, M., 2002. Mulberry Common. Online aveable: http://botanical.com/botanical/mgmh/m/mul.com62.html (01.05.2023).
- Huo, Y. (2002). Mulberry cultivation and utilization in China. Mulberry for animal production, FAO. Animal Production and Health Paper, 147, 11-44.
- Kovalchuk, I.Y., Mukhitdinova, Z., Turdiyev, T., Madiyeva, G., Akin, M., Eyduran, E., Reed, B.M. (2017). Modeling some mineral nutrient requirements for micropropagated wild apricot shoot cultures. Plant Cell, Tissue and Organ Culture, 129(2), 325-335.
- Machii, H., Koyama, A., Yamanouchi, H., Matsumoto K., Kobayashi, S., Katagiri, K. (2001). A list of morphological and agronomical traits of mulberry genetic resources. Misc. Publ. Natl. Inst. Seric. Entomol. Sci., 29, 1-307.
- Mahanta, P. K., Boruah, S.K., Boruah, H.K., Kalıta, N.J., 1993. Changes of poliphenol oxidase and peroxidase activites and pigment composition of some manufactured black teas (Camellia sinensis L.). Journal of Agriculture and Food Chemistry, 41, 272-276.
- Martin, G., Reyes, F., Hernandez, I., Milera, M. (2002). Agronomic studies with mulberry in Cuba. Mulberry for Animal Production, FAO Animal Production and Health Paper, 147, 103-114.
- MEB, 2013. http://megep.meb.gov.tr (09.04.2023).
- Rapeanu, G., Loey, A.V., Smouth, C., Hendrıckx, M. (2006). Biochemical characterization and process stability of poliphenoloxidase extacted from victoria grape (Vitis vinifera spp. sativa). Food Chemistry, 94, 253-261.
- Özbek, S. (1977). Genel Meyvecilik. Çukurova Üniversitesi Ziraat Fak., 111(6), Adana. 386.
- TUİK, 2014. Gıda, Tarım ve Hayvancılık Bakanlığı. https://www.tuik.gov.tr/ (12.05.2023).
- Türkoğlu, A., Bolouri, P., Haliloğlu, K., Eren, B., Demirel, F., et al. (2023a). Modeling callus induction and regeneration in hypocotyl explant of fodder pea (Pisum sativum var. arvense L.) using machine learning algorithm method. Agronomy, 13(11), 2835.
- Türkoğlu, A., Haliloğlu, K., Demirel, F., Aydin, M., Çiçek, S., et al. (2023b). Machine learning analysis of the ımpact of silver nitrate and silver nanoparticles on wheat (Triticum aestivum L.): Callus induction, plant regeneration, and DNA methylation. Plants, 12(24), 4151.
- Ünal, M. Ü. (2007). Properties of polyphenol oxidase from anamur banana (Musa cavendishii). Food Chemistry, 100(3), 909-913.
- Zheng, T., Tan, Y., Huang, G., Fan, H., Ma, B. (1988). Mulberry Cultivation. FAO Agriculturae Services Bulletin, 73/1, Rome, p127.
Details
| Primary Language | English |
|---|---|
| Subjects | Pomology and Treatment |
| Journal Section | Research Articles |
| Authors | |
| Early Pub Date | December 31, 2023 |
| Publication Date | December 31, 2023 |
| Submission Date | October 24, 2023 |
| Acceptance Date | December 29, 2023 |
| Published in Issue | Year 2023 Volume: 6 Issue: 2 |
