Background and Aims: A new sensitive and selective method for the determination of sultamicillin in bulk and pharmaceutical preparations has been developed. Methods: The developed method is based on the derivatization of sultamicillin with 4-chloro-7-nitrobenzofurazan in pH 9.0 borate buffer at 70ºC for 60 min. The derivative was measured spectrophotometrically at 432 nm after extraction with chloroform. Results: The method was validated by linearity, LOD, LOQ, accuracy, precision and recovery parameters. The linear range for sultamicillin was 10-50 µg.mL-1 with a correlation coefficient of 0.9999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.47 and 4.41 µg/mL-1, respectively. Conclusion: The suggested method was validated, and can be applied for routine sultamicillin analysis in dosage formsfor industrial analysis.
Primary Language | English |
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Subjects | Pharmacology and Pharmaceutical Sciences, Health Care Administration |
Journal Section | Original Article |
Authors | |
Publication Date | April 1, 2020 |
Submission Date | July 11, 2019 |
Published in Issue | Year 2020 Volume: 50 Issue: 1 |