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In vitro skin permeation of escin in the new gel formulation of Aesculus hippocastanum (Horse Chestnut)

Year 2016, Volume: 46 Issue: 2, 79 - 88, 26.12.2016

Esra Eroğlu Özkan Burcu Mesut Abdulselam Polat Nur Tan

Abstract

Aesculus hippocastanum L. (Hippocastanaceae) is used in topical pharmaceutical and cosmetic formulations by industries. The aim of this work is to develop a new A. hippocastanum product having good stability and better skin permeation compared with the other products on the market. Two new gel formulations were prepared to evaluate escin ex vivo permeation through pig skin by using Franz-type diffusion and to analyse by HPLC. The first gel formulation has contained only A. hippocastanum extract (G1) and the second gel formulation has contained a mixture of A. hippocastanum and M. chamomilla extracts (G2), as active ingredients. The reference gel has escin as active ingredient and it was purchased from a pharmacy. G2 showed significantly better skin permeation ability with good stability compared to G1 and reference gel. Owing to its favourable penetration and stability characteristics the new gel formulation could be an alternative for the treatment of chronic venous insufficiency and inflammatory soft tissue inflammation.

Keywords

Aesculus hippocastanum gel escin HPLC stability

References

  • Angoules AG (2015). Conservative Treatment of Chronic Venous Insufficiency J Nov Physiother 5: e135.
  • Aziz Z, Tang WL, Chong NJ, Tho LY (2015). A systematic review of the efficacy and tolerability of hydroxyethylrutosides for improvement of the signs and symptoms of chronic venous insufficiency J Clin Pharmacol 40: 177-185.
  • Baibado, Tarra J, Cheung HY (2010). Seed Extract of Horse Chestnut (Aesculus hippocastanum L.,) as effective medication for chronic venous insufficiency and other health benefits looking back on 2010: A Year Strived for A Higher Standard: 156.
  • Almeida D, Priscila A, Alves MC, Hudson CP, Dutra LS, Leite MN, Raposo NRB, Ferreira AO, Brandao MAF (2013). New HPLC method for quality control of β-escin in Aesculus hippocastanum L. hydroalcoholic extract Lat. Am. J. Pharm. 32: 1082-1087.
  • Belcaro G, Cesarone MR, Dugall M (2004). Microcirculatory efficacy of topical treatment with aescin+ essential phospholipids gel in venous insufficiency and hypertension: new clinical observations Angiology 55: S1-S5. Deok Hoon P, Lee JS, Hong ST, Hyun CG, Lee SY (2007). Natural composite preservatives In.: Google Patents.
  • Gupta V, Mittal P, Bansal P, Khokra SL, Kaushik D (2010). Pharmacological potential of Matricaria recutita-A review Int. J. Pharm. Sci. Drug Res. 2: 12-16.
  • Matsuda H, Li Y, Murakami T, Ninomiya K, Yamahara J, Yoshikawa M (1997). Effects of escins Ia, Ib, IIa, and IIb from horse chestnut, the seeds of Aesculus hippocastanum L., on acute inflammation in animals Biol Pharm Bull 20: 1092-1095.
  • Montenegro LCC, Giannone I, Puglisi G (2007). Use of solid phase extraction (SPE) to evaluate in vitro skin permeation of aescin Die Pharmazie 62: 342-345.
  • Ruffini IGB, Cesarone MR, Dugall M (2004). Efficacy of topical treatment with aescin+ essential phospholipids gel in venous insufficiency and hypertension Angiology 55: S19-S21.
  • Sirtori, Cesare R (2001). Aescin: pharmacology, pharmacokinetics and therapeutic profile. Pharmacol Res 44: 183-193.
  • Srivastava, Janmejai K, Shankar E, Gupta S (2010). Chamomile: A herbal medicine of the past with a bright future (Review) Mol Med Rep. 3: 895-901.
  • Suter A, Bommer S, Rechner J (2006). Treatment of patients with venous insufficiency with fresh plant horse chestnut seed extract: a review of 5 clinical studies Adv Ther 23: 179-190.
  • Wilkinson JA, Brown AMG (1999). Horse Chestnut–Aesculus Hippocastanum: Potential Applications in Cosmetic Skin care Products Int J Cosmetic Sci 21: 437-447.

Year 2016, Volume: 46 Issue: 2, 79 - 88, 26.12.2016

Esra Eroğlu Özkan Burcu Mesut Abdulselam Polat Nur Tan

Abstract

References

  • Angoules AG (2015). Conservative Treatment of Chronic Venous Insufficiency J Nov Physiother 5: e135.
  • Aziz Z, Tang WL, Chong NJ, Tho LY (2015). A systematic review of the efficacy and tolerability of hydroxyethylrutosides for improvement of the signs and symptoms of chronic venous insufficiency J Clin Pharmacol 40: 177-185.
  • Baibado, Tarra J, Cheung HY (2010). Seed Extract of Horse Chestnut (Aesculus hippocastanum L.,) as effective medication for chronic venous insufficiency and other health benefits looking back on 2010: A Year Strived for A Higher Standard: 156.
  • Almeida D, Priscila A, Alves MC, Hudson CP, Dutra LS, Leite MN, Raposo NRB, Ferreira AO, Brandao MAF (2013). New HPLC method for quality control of β-escin in Aesculus hippocastanum L. hydroalcoholic extract Lat. Am. J. Pharm. 32: 1082-1087.
  • Belcaro G, Cesarone MR, Dugall M (2004). Microcirculatory efficacy of topical treatment with aescin+ essential phospholipids gel in venous insufficiency and hypertension: new clinical observations Angiology 55: S1-S5. Deok Hoon P, Lee JS, Hong ST, Hyun CG, Lee SY (2007). Natural composite preservatives In.: Google Patents.
  • Gupta V, Mittal P, Bansal P, Khokra SL, Kaushik D (2010). Pharmacological potential of Matricaria recutita-A review Int. J. Pharm. Sci. Drug Res. 2: 12-16.
  • Matsuda H, Li Y, Murakami T, Ninomiya K, Yamahara J, Yoshikawa M (1997). Effects of escins Ia, Ib, IIa, and IIb from horse chestnut, the seeds of Aesculus hippocastanum L., on acute inflammation in animals Biol Pharm Bull 20: 1092-1095.
  • Montenegro LCC, Giannone I, Puglisi G (2007). Use of solid phase extraction (SPE) to evaluate in vitro skin permeation of aescin Die Pharmazie 62: 342-345.
  • Ruffini IGB, Cesarone MR, Dugall M (2004). Efficacy of topical treatment with aescin+ essential phospholipids gel in venous insufficiency and hypertension Angiology 55: S19-S21.
  • Sirtori, Cesare R (2001). Aescin: pharmacology, pharmacokinetics and therapeutic profile. Pharmacol Res 44: 183-193.
  • Srivastava, Janmejai K, Shankar E, Gupta S (2010). Chamomile: A herbal medicine of the past with a bright future (Review) Mol Med Rep. 3: 895-901.
  • Suter A, Bommer S, Rechner J (2006). Treatment of patients with venous insufficiency with fresh plant horse chestnut seed extract: a review of 5 clinical studies Adv Ther 23: 179-190.
  • Wilkinson JA, Brown AMG (1999). Horse Chestnut–Aesculus Hippocastanum: Potential Applications in Cosmetic Skin care Products Int J Cosmetic Sci 21: 437-447.
There are 13 citations in total.

Details

Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Araştırma Makalesi
Authors

Esra Eroğlu Özkan

Burcu Mesut

Abdulselam Polat This is me

Nur Tan This is me

Publication Date December 26, 2016
Published in Issue Year 2016 Volume: 46 Issue: 2

Cite

APA Eroğlu Özkan, E., Mesut, B., Polat, A., Tan, N. (2016). In vitro skin permeation of escin in the new gel formulation of Aesculus hippocastanum (Horse Chestnut). Journal of Faculty of Pharmacy of Istanbul University, 46(2), 79-88.
AMA Eroğlu Özkan E, Mesut B, Polat A, Tan N. In vitro skin permeation of escin in the new gel formulation of Aesculus hippocastanum (Horse Chestnut). Journal of Faculty of Pharmacy of Istanbul University. December 2016;46(2):79-88.
Chicago Eroğlu Özkan, Esra, Burcu Mesut, Abdulselam Polat, and Nur Tan. “In Vitro Skin Permeation of Escin in the New Gel Formulation of Aesculus Hippocastanum (Horse Chestnut)”. Journal of Faculty of Pharmacy of Istanbul University 46, no. 2 (December 2016): 79-88.
EndNote Eroğlu Özkan E, Mesut B, Polat A, Tan N (December 1, 2016) In vitro skin permeation of escin in the new gel formulation of Aesculus hippocastanum (Horse Chestnut). Journal of Faculty of Pharmacy of Istanbul University 46 2 79–88.
IEEE E. Eroğlu Özkan, B. Mesut, A. Polat, and N. Tan, “In vitro skin permeation of escin in the new gel formulation of Aesculus hippocastanum (Horse Chestnut)”, Journal of Faculty of Pharmacy of Istanbul University, vol. 46, no. 2, pp. 79–88, 2016.
ISNAD Eroğlu Özkan, Esra et al. “In Vitro Skin Permeation of Escin in the New Gel Formulation of Aesculus Hippocastanum (Horse Chestnut)”. Journal of Faculty of Pharmacy of Istanbul University 46/2 (December 2016), 79-88.
JAMA Eroğlu Özkan E, Mesut B, Polat A, Tan N. In vitro skin permeation of escin in the new gel formulation of Aesculus hippocastanum (Horse Chestnut). Journal of Faculty of Pharmacy of Istanbul University. 2016;46:79–88.
MLA Eroğlu Özkan, Esra et al. “In Vitro Skin Permeation of Escin in the New Gel Formulation of Aesculus Hippocastanum (Horse Chestnut)”. Journal of Faculty of Pharmacy of Istanbul University, vol. 46, no. 2, 2016, pp. 79-88.
Vancouver Eroğlu Özkan E, Mesut B, Polat A, Tan N. In vitro skin permeation of escin in the new gel formulation of Aesculus hippocastanum (Horse Chestnut). Journal of Faculty of Pharmacy of Istanbul University. 2016;46(2):79-88.
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