Research Article
BibTex RIS Cite

Chemical Composition of Taraxacum Officinale Extracts and Anti-Proliferative Effect on A549 and HT-29 Cell Lines Through Regulating the Expression of Apoptosis-Related Genes

Year 2024, Volume: 45 Issue: 4, 693 - 700, 30.12.2024

Serkan Kapancık

https://doi.org/10.17776/csj.1520045

Abstract

The use of medicinal plants is still of great importance as an alternative treatment method in neurodegenerative diseases, cardiovascular patients and cancer. Taraxacum officinale plant is one of the medical plants used in the treatment of many diseases as diuretic, hypolipidemic and antidiabetic thanks to the active components it contains. In order to examine the effect of Taraxacum officinal on proliferation and apoptosis in cancer, in our study, we determined the chemical composition of the Taraxacum officinal plant by gas chromatography-mass spectrometry (GC-MS) and its antioxidant status using a commercial kit. Additionally, we determined the anti-cancer effect of water, methanol and chloroform extracts of the Taraxacum officinal plant on A549 and HT-29 cancer cell lines with the help of the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) method. Finally, we aimed to determine the relationship between the chloroform extract of the Taraxacum officinal plant, which has the highest anti-cancer properties, and apoptosis by determining the expressions of BAX, BCL-2 and Caspase-3, which are among the genes associated with apoptosis, in A549 and HT-29 cancer cell lines, with the help of Real-time Polymerase Chain Reaction (RT-PCR) analysis. We determined that the Taraxacum officinal plant has an anti-cancer effect by increasing caspase-3 expressions in A549 lung cancer cell lines and by increasing caspase-3 and BAX expressions and decreasing BCL-2 expression in HT-29 colon cancer cell lines. In light of the data we obtained, we can say that the Taraxacum officinal plant has anti-cancer properties that cause a decrease in cell proliferation by regulating the expression of genes related to apoptosis

Keywords

Cancer GC-MS Taraxacum officinal Apoptosis Gene expression

References

  • [1] Petrovska B.B., Historical review of medicinal plants’ usage, Pharmacognosy Reviews, 6 (11) (2012) 1.
  • [2] Dutra R.C., Campos M.M., Santos A.R., Calixto J.B., Medicinal plants in Brazil: Pharmacological studies, drug discovery, challenges and perspectives, Pharmacological Research, 112 (2016) 4-29.
  • [3] Sarkar B., Rana N., Singh C., Singh A., Medicinal herbal remedies in neurodegenerative diseases: an update on antioxidant potential, Naunyn-Schmiedeberg's Archives of Pharmacology, (2024) 1-29.
  • [4] Tokhirovich, A.A., Maksudjanovna M.G., Phytopharmacokinetics of Medicinal Plants and Drug Preparations Affecting Cardiovascular Diseases, Web of Semantics: Journal of Interdisciplinary Science, 2 (2) (2024) 10-12.
  • [5] Gielecińska A., Kciuk M., Mujwar S., Celik I., Kołat D., Kałuzińska-Kołat Ż., Kontek R., Substances of natural origin in medicine: Plants vs. cancer, Cells, 12 (7) (2023) 986.
  • [6] Di Napoli A., Zucchetti P., A comprehensive review of the benefits of Taraxacum officinale on human health, Bulletin of the National Research Centre, 45 (1) (2021) 110.
  • [7] Clare B.A., Conroy R.S., Spelman K., The diuretic effect in human subjects of an extract of Taraxacum officinale folium over a single day, The Journal of Alternative and Complementary Medicine, 15 (8) (2009) 929-934.
  • [8] Mir M.A., Sawhney S.S., Jassal M.M.S., In-vitro antidiabetic studies of various extracts of Taraxacum officinale, The Pharma Innovation, 4 (1) (2015) 61.
  • [9] Choi U.K., Lee O.H., Yim J.H., Cho C.W., Rhee Y.K., Lim S.I., Kim Y.C., Hypolipidemic and antioxidant effects of dandelion (Taraxacum officinale) root and leaf on cholesterol-fed rabbits, International journal of molecular sciences, 11 (1) (2010) 67-78.
  • [10] Colle D., Arantes L.P., Gubert P., da Luz S.C.A., Athayde M.L., Teixeira Rocha J.B., Soares F.A.A., Antioxidant properties of Taraxacum officinale leaf extract are involved in the protective effect against hepatoxicity induced by acetaminophen in mice, Journal of medicinal food, 15 (6) (2012) 549-556.
  • [11] Sigstedt S.C., Hooten C.J., Callewaert M.C., Jenkins A.R., Romero A.E., Pullin M.J., ... & Steelant W.F., Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells, International journal of oncology, 32 (5) (2008) 1085-1090.
  • [12] Menke K., Schwermer M., Felenda J., Beckmann C., Stintzing F., Schramm A., Zuzak T.J., Taraxacum officinale extract shows antitumor effects on pediatric cancer cells and enhance mistletoe therapy, Complementary therapies in medicine, 40 (2018) 158-164.
  • [13] Ahmadi S., Saberivand A., Jalili C., Asadpour R., Khordadmehr M., Saberivand M., Hydroalcoholic extract of Taraxacum officinale induces apoptosis and autophagy in 4T1 breast cancer cells, In Veterinary Research Forum, 14 (9) (2023) 507.
  • [14] Letai A., Apoptosis and cancer, Annual Review of Cancer Biology, 1 (1) (2017) 275-294.
  • [15] Kiraz Y., Adan A., Kartal Yandim M., Baran Y., Major apoptotic mechanisms and genes involved in apoptosis, Tumor Biology, 37 (2016) 8471-8486.
  • [16] Kapancık S., Çelik M.S., Demiralp M., Ünal K., Çetinkaya S., Tüzün B., Chemical composition, cytotoxicity, and molecular docking analyses of Thuja orientalis extracts. Journal of Molecular Structure, 1318 (2024) 139279.
  • [17] Motafeghi F., Shahsavari R., Mortazavi P., Shokrzadeh M., Anticancer effect of paroxetine and amitriptyline on HT29 and A549 cell lines, Toxicology in Vitro, 87 (2023) 105532.
  • [18] Liu G., Wang T., Wang T., Song J., Zhou Z., Effects of apoptosis‑related proteins caspase‑3, Bax and Bcl‑2 on cerebral ischemia rats, Biomedical reports, 1 (6) (2013) 861-867.
  • [19] Erel O., A novel automated method to measure total antioxidant response against potent free radical reactions, Clinical biochemistry, 37 (2) (2004) 112-119.
  • [20] Kapancık S., Çelik V., Kılıçkap S., Kacan T., Kapancik S., The relationship of agmatine deficiency with the lung cancer, Uhod-Uluslararasi Hematoloji-Onkoloji Dergisi, 26 (2016) 103-109.
  • [21] Trinh N.V., Doan-Phuong Dang N., Hong Tran D., Van Pham P., Taraxacum officinale dandelion extracts efficiently inhibited the breast cancer stem cell proliferation, Biomedical Research and Therapy, 3 (2016) 1-13.
  • [22] Rawa’a A.M., Cytotoxic activity of taraxacum officinale ethanolic plant extract against human breast cancer (MCF-7) cells and human hepatic (WRL-68) cells, Iraqi Journal of Cancer and Medical Genetics, 11 (1) (2018).
  • [23] Choi E.J., Kim G.H., Dandelion (Taraxacum officinale) flower ethanol extract inhibits cell proliferation and induces apoptosis in human ovarian cancer SK-OV-3 cells, Food science and biotechnology, 18 (2) (2009) 552-555.
  • [24] Fulga A., Casian A., Casian I., Protopop S., Gudumac V., Tagadiuc O., Effects of Taraxacum officinale on glioblastoma cell culture and their correlation with hydroxycinnamic acids content, In Medical Sciences Forum, 21 (1) (2023) 18.
  • [25] Fulga A., Todiras M., Gudumac V., Tagadiuc O., Taraxacum officinale leaves and roots suppress glioma cell viability, Journal of Biosciences and Medicines, 10 (3) (2022) 175-189.
  • [26] Kasthuri K., Rajkumar P., Ranchani A.A.J., Jayaprakash K., Bio-inspired synthesis of ZnO nanoparticles using Taraxacum officinale for antibacterial, antifungal, and anticancer applications, Inorganic Chemistry Communications, 170 (3) (2024) 113409.
  • [27] Korbášová M., Tomenendálová J., Chloupek J., Anti-tumour effect of combinations of three acids isolated from Taraxacum officinale, Acta Veterinaria Brno, 91 (1) (2022) 77-85.
  • [28] Kamal F.Z., Lefter R., Mihai C.T., Farah H., Ciobica A., Ali A., ... & Ech-Chahad A., Chemical composition, antioxidant and antiproliferative activities of Taraxacum officinale essential oil, Molecules, 27 (19) (2022) 6477.
  • [29] Yoon H.S., An H., Park C.M., Anti-metastatic Effect of Taraxacum Officinale Water and Ethanol Extracts Through the Regulation of Epithelial-Mesenchymal Transition in Huh7 Cells, Journal of The Korean Society of Integrative Medicine, 11 (3) (2023) 59-67.
  • [30] Al-Eisawi Z., Abderrahman S.M., Al-Khalaf I.F., Al-Abbassi R., Bustanji Y.K., Taraxacum officinale extracts exhibit safe and selective anticancer activity, The Natural Products Journal, 12 (1) (2022) 69-77.
  • [31] Venezuela R.F., Mosmann J.P., Mugas M.L., Kiguen A.X., Montoya S.C.N., Konigheim B.S., Cuffini C.G., Dandelion Root Extract Affects the Proliferation, Survival and Migration of Cervical Cancer Cell Lines, Research Square, (2021) 1-15.
  • [32] Appiah-Opong R., Tuffour I., Annor G.K., Doris A., Blankson-Darku P.C., Kissi-Twum A.A., ... & Ocloo A., Antiproliferative, antioxidant activities and apoptosis induction by Morinda lucida and Taraxacum officinale in human HL-60 leukemia cells, Journal of Global Biosciences, 5 (7) (2016) 4281-4291.
  • [33] Erdem M., Özaslan M., Investigation of the antioxidant capacity of Taraxacum officinale l. leaf extract, The Eurasia Proceedings of Science Technology Engineering and Mathematics, 28, (2024) 575-580.
  • [34] Dedić S., Džaferović A., Jukić H., Chemical Composition and Antioxidant Activity of Water-Ethanol Extracts of Dandelion (Taraxacum officinale), Hrana u zdravlju i bolesti: znanstveno-stručni časopis za nutricionizam i dijetetiku, 11 (1) (2022) 8-14.
  • [35] Akhtar W., Ali G., Ashraf N., Fatima I., Kayani W.K., Shaheen H., ... & Khames A., Efficiency of Multiple Extraction Solvents on Antioxidant, Cytotoxic, and Phytotoxic Potential of Taraxacum officinale (L.) Weber ex FH Wigg. from Poonch Valley, Azad Kashmir, Pakistan, Evidence‐Based Complementary and Alternative Medicine, 1 (2022) 5118553.
  • [36] Daniel I.E., Mathew K.N., John P.L., Evaluation of vitamin contents, antioxidant and antimicrobial activities of different leaf extracts of Taraxacum officinale (dandelion), Journal of Complementary and Alternative Medical Research, 13 (1) (2021) 13-26.
  • [37] Lis B., Jedrejek D., Rywaniak J., Soluch A., Stochmal A., Olas B., Flavonoid preparations from Taraxacum officinale L. fruits—A phytochemical, antioxidant and hemostasis studies, Molecules, 25 (22) (2020) 5402.

Year 2024, Volume: 45 Issue: 4, 693 - 700, 30.12.2024

Serkan Kapancık

https://doi.org/10.17776/csj.1520045

Abstract

References

  • [1] Petrovska B.B., Historical review of medicinal plants’ usage, Pharmacognosy Reviews, 6 (11) (2012) 1.
  • [2] Dutra R.C., Campos M.M., Santos A.R., Calixto J.B., Medicinal plants in Brazil: Pharmacological studies, drug discovery, challenges and perspectives, Pharmacological Research, 112 (2016) 4-29.
  • [3] Sarkar B., Rana N., Singh C., Singh A., Medicinal herbal remedies in neurodegenerative diseases: an update on antioxidant potential, Naunyn-Schmiedeberg's Archives of Pharmacology, (2024) 1-29.
  • [4] Tokhirovich, A.A., Maksudjanovna M.G., Phytopharmacokinetics of Medicinal Plants and Drug Preparations Affecting Cardiovascular Diseases, Web of Semantics: Journal of Interdisciplinary Science, 2 (2) (2024) 10-12.
  • [5] Gielecińska A., Kciuk M., Mujwar S., Celik I., Kołat D., Kałuzińska-Kołat Ż., Kontek R., Substances of natural origin in medicine: Plants vs. cancer, Cells, 12 (7) (2023) 986.
  • [6] Di Napoli A., Zucchetti P., A comprehensive review of the benefits of Taraxacum officinale on human health, Bulletin of the National Research Centre, 45 (1) (2021) 110.
  • [7] Clare B.A., Conroy R.S., Spelman K., The diuretic effect in human subjects of an extract of Taraxacum officinale folium over a single day, The Journal of Alternative and Complementary Medicine, 15 (8) (2009) 929-934.
  • [8] Mir M.A., Sawhney S.S., Jassal M.M.S., In-vitro antidiabetic studies of various extracts of Taraxacum officinale, The Pharma Innovation, 4 (1) (2015) 61.
  • [9] Choi U.K., Lee O.H., Yim J.H., Cho C.W., Rhee Y.K., Lim S.I., Kim Y.C., Hypolipidemic and antioxidant effects of dandelion (Taraxacum officinale) root and leaf on cholesterol-fed rabbits, International journal of molecular sciences, 11 (1) (2010) 67-78.
  • [10] Colle D., Arantes L.P., Gubert P., da Luz S.C.A., Athayde M.L., Teixeira Rocha J.B., Soares F.A.A., Antioxidant properties of Taraxacum officinale leaf extract are involved in the protective effect against hepatoxicity induced by acetaminophen in mice, Journal of medicinal food, 15 (6) (2012) 549-556.
  • [11] Sigstedt S.C., Hooten C.J., Callewaert M.C., Jenkins A.R., Romero A.E., Pullin M.J., ... & Steelant W.F., Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells, International journal of oncology, 32 (5) (2008) 1085-1090.
  • [12] Menke K., Schwermer M., Felenda J., Beckmann C., Stintzing F., Schramm A., Zuzak T.J., Taraxacum officinale extract shows antitumor effects on pediatric cancer cells and enhance mistletoe therapy, Complementary therapies in medicine, 40 (2018) 158-164.
  • [13] Ahmadi S., Saberivand A., Jalili C., Asadpour R., Khordadmehr M., Saberivand M., Hydroalcoholic extract of Taraxacum officinale induces apoptosis and autophagy in 4T1 breast cancer cells, In Veterinary Research Forum, 14 (9) (2023) 507.
  • [14] Letai A., Apoptosis and cancer, Annual Review of Cancer Biology, 1 (1) (2017) 275-294.
  • [15] Kiraz Y., Adan A., Kartal Yandim M., Baran Y., Major apoptotic mechanisms and genes involved in apoptosis, Tumor Biology, 37 (2016) 8471-8486.
  • [16] Kapancık S., Çelik M.S., Demiralp M., Ünal K., Çetinkaya S., Tüzün B., Chemical composition, cytotoxicity, and molecular docking analyses of Thuja orientalis extracts. Journal of Molecular Structure, 1318 (2024) 139279.
  • [17] Motafeghi F., Shahsavari R., Mortazavi P., Shokrzadeh M., Anticancer effect of paroxetine and amitriptyline on HT29 and A549 cell lines, Toxicology in Vitro, 87 (2023) 105532.
  • [18] Liu G., Wang T., Wang T., Song J., Zhou Z., Effects of apoptosis‑related proteins caspase‑3, Bax and Bcl‑2 on cerebral ischemia rats, Biomedical reports, 1 (6) (2013) 861-867.
  • [19] Erel O., A novel automated method to measure total antioxidant response against potent free radical reactions, Clinical biochemistry, 37 (2) (2004) 112-119.
  • [20] Kapancık S., Çelik V., Kılıçkap S., Kacan T., Kapancik S., The relationship of agmatine deficiency with the lung cancer, Uhod-Uluslararasi Hematoloji-Onkoloji Dergisi, 26 (2016) 103-109.
  • [21] Trinh N.V., Doan-Phuong Dang N., Hong Tran D., Van Pham P., Taraxacum officinale dandelion extracts efficiently inhibited the breast cancer stem cell proliferation, Biomedical Research and Therapy, 3 (2016) 1-13.
  • [22] Rawa’a A.M., Cytotoxic activity of taraxacum officinale ethanolic plant extract against human breast cancer (MCF-7) cells and human hepatic (WRL-68) cells, Iraqi Journal of Cancer and Medical Genetics, 11 (1) (2018).
  • [23] Choi E.J., Kim G.H., Dandelion (Taraxacum officinale) flower ethanol extract inhibits cell proliferation and induces apoptosis in human ovarian cancer SK-OV-3 cells, Food science and biotechnology, 18 (2) (2009) 552-555.
  • [24] Fulga A., Casian A., Casian I., Protopop S., Gudumac V., Tagadiuc O., Effects of Taraxacum officinale on glioblastoma cell culture and their correlation with hydroxycinnamic acids content, In Medical Sciences Forum, 21 (1) (2023) 18.
  • [25] Fulga A., Todiras M., Gudumac V., Tagadiuc O., Taraxacum officinale leaves and roots suppress glioma cell viability, Journal of Biosciences and Medicines, 10 (3) (2022) 175-189.
  • [26] Kasthuri K., Rajkumar P., Ranchani A.A.J., Jayaprakash K., Bio-inspired synthesis of ZnO nanoparticles using Taraxacum officinale for antibacterial, antifungal, and anticancer applications, Inorganic Chemistry Communications, 170 (3) (2024) 113409.
  • [27] Korbášová M., Tomenendálová J., Chloupek J., Anti-tumour effect of combinations of three acids isolated from Taraxacum officinale, Acta Veterinaria Brno, 91 (1) (2022) 77-85.
  • [28] Kamal F.Z., Lefter R., Mihai C.T., Farah H., Ciobica A., Ali A., ... & Ech-Chahad A., Chemical composition, antioxidant and antiproliferative activities of Taraxacum officinale essential oil, Molecules, 27 (19) (2022) 6477.
  • [29] Yoon H.S., An H., Park C.M., Anti-metastatic Effect of Taraxacum Officinale Water and Ethanol Extracts Through the Regulation of Epithelial-Mesenchymal Transition in Huh7 Cells, Journal of The Korean Society of Integrative Medicine, 11 (3) (2023) 59-67.
  • [30] Al-Eisawi Z., Abderrahman S.M., Al-Khalaf I.F., Al-Abbassi R., Bustanji Y.K., Taraxacum officinale extracts exhibit safe and selective anticancer activity, The Natural Products Journal, 12 (1) (2022) 69-77.
  • [31] Venezuela R.F., Mosmann J.P., Mugas M.L., Kiguen A.X., Montoya S.C.N., Konigheim B.S., Cuffini C.G., Dandelion Root Extract Affects the Proliferation, Survival and Migration of Cervical Cancer Cell Lines, Research Square, (2021) 1-15.
  • [32] Appiah-Opong R., Tuffour I., Annor G.K., Doris A., Blankson-Darku P.C., Kissi-Twum A.A., ... & Ocloo A., Antiproliferative, antioxidant activities and apoptosis induction by Morinda lucida and Taraxacum officinale in human HL-60 leukemia cells, Journal of Global Biosciences, 5 (7) (2016) 4281-4291.
  • [33] Erdem M., Özaslan M., Investigation of the antioxidant capacity of Taraxacum officinale l. leaf extract, The Eurasia Proceedings of Science Technology Engineering and Mathematics, 28, (2024) 575-580.
  • [34] Dedić S., Džaferović A., Jukić H., Chemical Composition and Antioxidant Activity of Water-Ethanol Extracts of Dandelion (Taraxacum officinale), Hrana u zdravlju i bolesti: znanstveno-stručni časopis za nutricionizam i dijetetiku, 11 (1) (2022) 8-14.
  • [35] Akhtar W., Ali G., Ashraf N., Fatima I., Kayani W.K., Shaheen H., ... & Khames A., Efficiency of Multiple Extraction Solvents on Antioxidant, Cytotoxic, and Phytotoxic Potential of Taraxacum officinale (L.) Weber ex FH Wigg. from Poonch Valley, Azad Kashmir, Pakistan, Evidence‐Based Complementary and Alternative Medicine, 1 (2022) 5118553.
  • [36] Daniel I.E., Mathew K.N., John P.L., Evaluation of vitamin contents, antioxidant and antimicrobial activities of different leaf extracts of Taraxacum officinale (dandelion), Journal of Complementary and Alternative Medical Research, 13 (1) (2021) 13-26.
  • [37] Lis B., Jedrejek D., Rywaniak J., Soluch A., Stochmal A., Olas B., Flavonoid preparations from Taraxacum officinale L. fruits—A phytochemical, antioxidant and hemostasis studies, Molecules, 25 (22) (2020) 5402.
There are 37 citations in total.

Details

Primary Language English
Subjects Cancer Biology
Journal Section Natural Sciences
Authors

Serkan Kapancık 0000-0003-3019-4275

Publication Date December 30, 2024
Submission Date July 22, 2024
Acceptance Date December 4, 2024
Published in Issue Year 2024Volume: 45 Issue: 4

Cite

APA Kapancık, S. (2024). Chemical Composition of Taraxacum Officinale Extracts and Anti-Proliferative Effect on A549 and HT-29 Cell Lines Through Regulating the Expression of Apoptosis-Related Genes. Cumhuriyet Science Journal, 45(4), 693-700. https://doi.org/10.17776/csj.1520045
 Download Cover Image