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Comparative Evaluation of Chemical Compositions and Antioxidant Activities of Passiflora edulis Sims Extracts

Year 2024, Volume: 45 Issue: 4, 658 - 662, 30.12.2024

Emine Kılıçkaya Selvi

https://doi.org/10.17776/csj.1507756

Abstract

Passiflora members have an important quality in both conventional and modern medicine. In the present study Passiflora edulis Sims from MediterraneanTurkey (Antalya-Gazipaşa) was investigated from the aspects of phenolic compounds and antioxidant activities The plant material (stems, leaves) used as a whole and chloroform, ethyl acetate and methanol were used for the extraction. High performance liquid chromatography was used to analyse the phenolic compounds of the extracts. Besides, the antioxidant potential of the extracts was characterised by the total phenolic content, the total flavonoid content, the DPPH (2,2-diphenyl-1-picrylhydrazyl), the CUPRAC (Cupric Ion Reducing Antioxidant Capacity) and the FRAP (ferric reducing antioxidant power assay) tests. Methanol extract was found to be more active than the other extracts in the all antioxidant experiment. DPPH radical scavenging activity of methanol extract was 0.028 ± 0.001 mg/mL, FPAP assay was 0.511 ± 0.012 µmolGA/g and CUPRAC assay was 3.728 ± 0.150 µmolGA/g. As a result, methanol extract of Passiflora edulis was established ferulic acid (175.12 mg std/g) and p-coumaric acid (116.88 mg std/g) were determined as primary phenolic compounds in different amounts along with protocatechuic acid, chlorogenic acid, caffeic acid, apigenin and isorhanmetin. The results showed that Passiflora edulis deserves to be studied both for its use as a food and for its therapeutic properties.

Keywords

Antioxidant activity HPLC-DAD Passiflora edulis Turkey

Ethical Statement

The author declare that they have no conflicts of interest.

Thanks

The author would like to thank Erciyes University, Drug Application and Research Center (ERFARMA).

References

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  • [8] Ingale S.P., Kasture S.B., Antioxidant and Antiparkinsonian Activity of Passiflora incarnata Leaves, Orient Pharm. Exp. Med., 14(3) (2014) 231–6.
  • [9] Uzunoğlu F., Özmen K., Toprak S., et al. The Effect of Different Pre-sowing Treatments on Seedling Emergence, Quality and Development in Passiflora edulis Seeds, Erwerbs-Obstbau., 65 (2023) 2509–2516.
  • [10] Alves J.S., Marques J.I., Demarque D.P., Costa L.R., Amaral J.G., Lopes N.P., da Silva-Júnior A.A., Soares L.A.L., Gavioli E.C., Ferreira L.S., Zucolotto S.M., Involvement of Isoorientin in the Antidepressant Bioactivity of a Flavonoid-rich Extract From Passiflora edulis f. flavicarpa Leaves, Rev. Bras. Farmacogn., 30 (2020) 1–11.
  • [11] Alvarez-Rivera G., Ballesteros-Vivas D., Parada-Alfonso F., Ibañez E., Cifuentes A., Recent Applications of High Resolution Mass Spectrometry for the Characterization of Plant Natural Products, TrAC Trends Anal. Chem., 112 (2019) 87–101.
  • [12] Ballesteros-Vivas D., Alvarez-Rivera G., Ibánez E., Parada- Alfonso F., Cifuentes A., Integrated Strategy for the Extraction and Profiling of Bioactive Metabolites from Passiflora mollissima seeds Combining Pressurized-liquid Extraction and Gas/liquid Chromatography–high Resolution Mass Spectrometry, J. Chromatogr. A., 1595 (2019) 144–57.
  • [13] Rodríguez A.J., Arteaga J.M., Arango W.M., Pabon M.F., Vasodilator Effect of Ethanolic Extracts of Passiflora vitifolia and Passiflora edulis f. edulis Seeds, J. Appl. Pharm. Sci., 11(10) (2021) 61-69.
  • [14] Viganó J., de Paula Assis B.F., Náthia-Neves G., dos Santos P., Meireles M.A.A., Veggi P.C., Martínez J., Extraction of Bioactive Compounds from Defatted Passion Fruit Bagasse (Passiflora edulis sp.) Applying Pressurized Liquids Assisted by Ultrasound, Ultrason Sonochem., 64 (2020) 104999.
  • [15] Aguillón J., Maldonado M., Loango N., Arango S., Landázuri P., Actividad Antioxidante Antiproliferativa de Extractos Etanólicos y Acuoso de las Hojas y el Jugo del Fruto de Passiflora edulis, Perspect Nutr. Hum., 15 (1) (2013) 13.
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  • [17] Villada Ramos J.A., Aguillón Osma J., Restrepo Cortes B., Loango Chamarro N., Maldonado Celis M.E., Identification of Potential Bioactive Compounds of Passiflora edulis Leaf Extract Against Colon Adenocarcinoma Cells, Biochem. Biophys. Rep., 28 (2023) 34-101453.
  • [18] Zibadi S., Watson R.R., Passion Fruit (Passiflora edulis) Composition, Efficacy and Safety, J. Evid. Based Integr. Med., 1 (2024) 183-187.
  • [19] Selvi E.K., Turumtay H., Demir A., Turumtay E.A., Phytochemical Profiling and Evaluation of the Hepatoprotective Effect of Cuscuta campestris by High-performance Liquid Chromatography with Diode Array Detection, Anal. Lett., 51 (10) (2018) 1464-1478.
  • [20] Yao L., Jiang Y., Datta N., Singanusong R., Liu X., Duan J., Raymont K., Lisle A., Xu Y., HPLC Analyses of Flavanols and Phenolic Acids in the Fresh Young Shoots of Tea (Camellia sinensis) Grown in Australia, Food Chem., 84 (2004) 253–63.
  • [21] Dmitrienko S.G., Kudrinskaya V.A., Apyari V.V., Methods of Extraction, Preconcentration, and Determination of Quercetin, J. Anal. Chem., 67(4) (2012) 299–311.
  • [22] Mokrani A., Madani K., Effect of Solvent, Time and Temperature on the Extraction of Phenolic Compounds and Antioxidant Capacity of Peach (Prunus persica L.) Fruit, Sep. Purif. Technol., 162 (2016) 68–76.
  • [23] Sukeksi L., Sarah M., Characterizations and Extraction of Polyphenols from Residual Pulp of Pink guava as Source of Antioxidants, J. Appl. Eng. Sci., 11 (2016) 5209–16.
  • [24] Stalikas C.D. Extraction, Separation, and Detection Methods for Phenolic acids and Flavonoids, J. Sep. Sci., 30 (2007) 3268–3295. doi: 10.1002/jssc.200700261.
  • [25] Selvi E.K., Güler N., Güven S., Makbul S., Determination of Chemical Compositions, Antioxidant, Dna Cleavage and Binding Properties of Vincetoxicum tmoleum Extract, Studia UBB Chemia, 1 (2024) 163-174.
  • [26] Singleton V.L., Orthofer R., Lamuela R.M., Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-ciocalteu Reagent, Methods Enzymol., 299 (1999) 152-178.
  • [27] Marcucci M., Woisky R., Salatino A., Uso de Cloreto de Alumínio na Quantificação de Flavonóides em Amostras de Própolis, Mensagem Doce., 46 (1998) 3-9.
  • [28] Molyneux P., The Use of the Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity, SJST., 26 (2) (2004) 211–19.
  • [29] Benzie I.F.F., Strain J.J., Ferric Reducing/Antioxidant Power Assay: Direct Measure of Total Antioxidant Activity of Biological Fluids and Modified Version for Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration, Meth. Enzymol., 299 (1999) 15–27.
  • [30] Apak R., Guclu K., Ozyurek M., Karademir SE., Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupricionreducing Capability in the Presence of Neocuproine: CUPRAC method, J. Agric. Food Chem., 52 (26) (2004) 7970-7981.
  • [31] Lourith N., Kanlayavattanakul M., Antioxidant Activities and Phenolics of Passiflora edulis Seed Recovered From Juice Production Residue, J. Oleo Sci., 62(4) (2013) 235-240.
  • [32] Sova M., Sosa L., Natural Sources, Pharmacokinetics, Biological Activities and Health Benefits of Hydroxycinnamic Acids and Their Metabolites, Nutrients, 12(8) (2020) 2190.
  • [33] Rotta E.M., Rodrigues C.A., Jardim I., Maldaner L., Visentainer J.V., Determination of Phenolic Compounds and Antioxidant Activity in Passion Fruit Pulp (Passiflora spp.) Using a modified QuEChERS Method and UHPLC-MS/MS, LWT., 100 (2019) 397-403.
  • [34] Santos T.B., Araujo F.P., Neto A.F., Freitas S.T., Souza A.J., Vilar O.S.B., Araújo A.J.B., Lima M.S., Phytochemical Compounds and Antioxidant Activity of the Pulp of Two Brazilian Passion Fruit Species: Passiflora cincinnata Mast. And Passiflora edulis Sims, Int. J. Fruit Sci., 21(1) (2021) 255-269.
  • [35] Colomeu T.C., Figueiredo D., Zollner R.L., Comparison of Antioxidant and Antiproliferative Effect Among Four Passiflora Spp, JALS., 4 (2) (2017) 1

Year 2024, Volume: 45 Issue: 4, 658 - 662, 30.12.2024

Emine Kılıçkaya Selvi

https://doi.org/10.17776/csj.1507756

Abstract

References

  • [1] Sevindik M., Mohammed F.S., Uysal I., Autism: Plants With Neuro-Psychopharmacotherapeutic Potential, Prospect. Pharm. Sci., 21(3) (2023) 38-48.
  • [2] El-Chaghaby G.A., Mohammed F.S., Rashad S., Uysal I., Koçer O., Lekesiz Ö., Sevindik M., Genus Hypericum: General Properties, Chemical Contents and Biological Activities, Egypt. J. Bot., 64(1) (2024) 1-26.
  • [3] Moloney J.N., Cotter T.G., ROS Signalling in the Biology of Cancer, Semin Cell Dev. Biol., 80 (2018) 50–64.
  • [4] Khan A.Q., Rashid K., AlAmodi A.A., Agha M.V., Akhtar S., Hakeem I., Raza S.S., Uddin S., Reactive Oxygen Species (ROS) in Cancer Pathogenesis and Therapy: An Update on the Role of ROS in Anticancer Action of Benzophenanthridine Alkaloids, Biomed. Pharmacother.,143 (2021) 112142.
  • [5] Ayres A.S., Araujo A.L., Soares T.C., et al. Comparative Central Effects of the Aqueous Leaf Extract of Two Populations of Passiflora edulis, Rev. Bras. Farmacogn., 25 (2015) 499-505.
  • [6] Wosch L., Santos K.C., Imig D.C., Santos C.A.M., Comparative Study of Passiflora Taxa Leaves: II. A Chromatographic Profile, Rev. Bras. farmacogn., 27 (2017) 40-49.
  • [7] Dhavan K., Dhavan S., Sharma A., Passiflora: a Review Update, J. Ethanopharmacol., 94 (2004) 1-23.
  • [8] Ingale S.P., Kasture S.B., Antioxidant and Antiparkinsonian Activity of Passiflora incarnata Leaves, Orient Pharm. Exp. Med., 14(3) (2014) 231–6.
  • [9] Uzunoğlu F., Özmen K., Toprak S., et al. The Effect of Different Pre-sowing Treatments on Seedling Emergence, Quality and Development in Passiflora edulis Seeds, Erwerbs-Obstbau., 65 (2023) 2509–2516.
  • [10] Alves J.S., Marques J.I., Demarque D.P., Costa L.R., Amaral J.G., Lopes N.P., da Silva-Júnior A.A., Soares L.A.L., Gavioli E.C., Ferreira L.S., Zucolotto S.M., Involvement of Isoorientin in the Antidepressant Bioactivity of a Flavonoid-rich Extract From Passiflora edulis f. flavicarpa Leaves, Rev. Bras. Farmacogn., 30 (2020) 1–11.
  • [11] Alvarez-Rivera G., Ballesteros-Vivas D., Parada-Alfonso F., Ibañez E., Cifuentes A., Recent Applications of High Resolution Mass Spectrometry for the Characterization of Plant Natural Products, TrAC Trends Anal. Chem., 112 (2019) 87–101.
  • [12] Ballesteros-Vivas D., Alvarez-Rivera G., Ibánez E., Parada- Alfonso F., Cifuentes A., Integrated Strategy for the Extraction and Profiling of Bioactive Metabolites from Passiflora mollissima seeds Combining Pressurized-liquid Extraction and Gas/liquid Chromatography–high Resolution Mass Spectrometry, J. Chromatogr. A., 1595 (2019) 144–57.
  • [13] Rodríguez A.J., Arteaga J.M., Arango W.M., Pabon M.F., Vasodilator Effect of Ethanolic Extracts of Passiflora vitifolia and Passiflora edulis f. edulis Seeds, J. Appl. Pharm. Sci., 11(10) (2021) 61-69.
  • [14] Viganó J., de Paula Assis B.F., Náthia-Neves G., dos Santos P., Meireles M.A.A., Veggi P.C., Martínez J., Extraction of Bioactive Compounds from Defatted Passion Fruit Bagasse (Passiflora edulis sp.) Applying Pressurized Liquids Assisted by Ultrasound, Ultrason Sonochem., 64 (2020) 104999.
  • [15] Aguillón J., Maldonado M., Loango N., Arango S., Landázuri P., Actividad Antioxidante Antiproliferativa de Extractos Etanólicos y Acuoso de las Hojas y el Jugo del Fruto de Passiflora edulis, Perspect Nutr. Hum., 15 (1) (2013) 13.
  • [16] Silva J.K., Cazarin C.B., Batista Â.G., Maróstica M., Effects of Passion Fruit (Passiflora edulis) Byproduct Intake in Antioxidant Status of Wistar Rats Tissues, LWT Food Sci. Technol., 59 (2) (2014) 1213-1219.
  • [17] Villada Ramos J.A., Aguillón Osma J., Restrepo Cortes B., Loango Chamarro N., Maldonado Celis M.E., Identification of Potential Bioactive Compounds of Passiflora edulis Leaf Extract Against Colon Adenocarcinoma Cells, Biochem. Biophys. Rep., 28 (2023) 34-101453.
  • [18] Zibadi S., Watson R.R., Passion Fruit (Passiflora edulis) Composition, Efficacy and Safety, J. Evid. Based Integr. Med., 1 (2024) 183-187.
  • [19] Selvi E.K., Turumtay H., Demir A., Turumtay E.A., Phytochemical Profiling and Evaluation of the Hepatoprotective Effect of Cuscuta campestris by High-performance Liquid Chromatography with Diode Array Detection, Anal. Lett., 51 (10) (2018) 1464-1478.
  • [20] Yao L., Jiang Y., Datta N., Singanusong R., Liu X., Duan J., Raymont K., Lisle A., Xu Y., HPLC Analyses of Flavanols and Phenolic Acids in the Fresh Young Shoots of Tea (Camellia sinensis) Grown in Australia, Food Chem., 84 (2004) 253–63.
  • [21] Dmitrienko S.G., Kudrinskaya V.A., Apyari V.V., Methods of Extraction, Preconcentration, and Determination of Quercetin, J. Anal. Chem., 67(4) (2012) 299–311.
  • [22] Mokrani A., Madani K., Effect of Solvent, Time and Temperature on the Extraction of Phenolic Compounds and Antioxidant Capacity of Peach (Prunus persica L.) Fruit, Sep. Purif. Technol., 162 (2016) 68–76.
  • [23] Sukeksi L., Sarah M., Characterizations and Extraction of Polyphenols from Residual Pulp of Pink guava as Source of Antioxidants, J. Appl. Eng. Sci., 11 (2016) 5209–16.
  • [24] Stalikas C.D. Extraction, Separation, and Detection Methods for Phenolic acids and Flavonoids, J. Sep. Sci., 30 (2007) 3268–3295. doi: 10.1002/jssc.200700261.
  • [25] Selvi E.K., Güler N., Güven S., Makbul S., Determination of Chemical Compositions, Antioxidant, Dna Cleavage and Binding Properties of Vincetoxicum tmoleum Extract, Studia UBB Chemia, 1 (2024) 163-174.
  • [26] Singleton V.L., Orthofer R., Lamuela R.M., Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-ciocalteu Reagent, Methods Enzymol., 299 (1999) 152-178.
  • [27] Marcucci M., Woisky R., Salatino A., Uso de Cloreto de Alumínio na Quantificação de Flavonóides em Amostras de Própolis, Mensagem Doce., 46 (1998) 3-9.
  • [28] Molyneux P., The Use of the Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity, SJST., 26 (2) (2004) 211–19.
  • [29] Benzie I.F.F., Strain J.J., Ferric Reducing/Antioxidant Power Assay: Direct Measure of Total Antioxidant Activity of Biological Fluids and Modified Version for Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration, Meth. Enzymol., 299 (1999) 15–27.
  • [30] Apak R., Guclu K., Ozyurek M., Karademir SE., Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupricionreducing Capability in the Presence of Neocuproine: CUPRAC method, J. Agric. Food Chem., 52 (26) (2004) 7970-7981.
  • [31] Lourith N., Kanlayavattanakul M., Antioxidant Activities and Phenolics of Passiflora edulis Seed Recovered From Juice Production Residue, J. Oleo Sci., 62(4) (2013) 235-240.
  • [32] Sova M., Sosa L., Natural Sources, Pharmacokinetics, Biological Activities and Health Benefits of Hydroxycinnamic Acids and Their Metabolites, Nutrients, 12(8) (2020) 2190.
  • [33] Rotta E.M., Rodrigues C.A., Jardim I., Maldaner L., Visentainer J.V., Determination of Phenolic Compounds and Antioxidant Activity in Passion Fruit Pulp (Passiflora spp.) Using a modified QuEChERS Method and UHPLC-MS/MS, LWT., 100 (2019) 397-403.
  • [34] Santos T.B., Araujo F.P., Neto A.F., Freitas S.T., Souza A.J., Vilar O.S.B., Araújo A.J.B., Lima M.S., Phytochemical Compounds and Antioxidant Activity of the Pulp of Two Brazilian Passion Fruit Species: Passiflora cincinnata Mast. And Passiflora edulis Sims, Int. J. Fruit Sci., 21(1) (2021) 255-269.
  • [35] Colomeu T.C., Figueiredo D., Zollner R.L., Comparison of Antioxidant and Antiproliferative Effect Among Four Passiflora Spp, JALS., 4 (2) (2017) 1
There are 35 citations in total.

Details

Primary Language English
Subjects Ethobotany, Metabolomic Chemistry, Natural Products and Bioactive Compounds
Journal Section Natural Sciences
Authors

Emine Kılıçkaya Selvi 0000-0003-0291-5362

Publication Date December 30, 2024
Submission Date June 30, 2024
Acceptance Date December 3, 2024
Published in Issue Year 2024Volume: 45 Issue: 4

Cite

APA Kılıçkaya Selvi, E. (2024). Comparative Evaluation of Chemical Compositions and Antioxidant Activities of Passiflora edulis Sims Extracts. Cumhuriyet Science Journal, 45(4), 658-662. https://doi.org/10.17776/csj.1507756
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