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Examination of Biological Activity of Passiflora edulis (Çarkıfelek) Extract via Phytochemical Analysis

Year 2024, Volume: 45 Issue: 3, 465 - 470, 30.09.2024

Tutku Tunç , Zeynep Sümer , Kerim Emre Öksüz

https://doi.org/10.17776/csj.1519699

Abstract

In recent years, the use of medicinal plants as sources of drugs or herbal extracts has been of great importance. Passiflora edulis is nowadays widely studied for its antimicrobial, anticancer, and antioxidant potential. Therefore, this study aimed to determine the phytochemical structure of ethanol extract of P. edulis leaves and to investigate its biological properties such as antimicrobial and anticancer activities. The ethanol extract of P. edulis leaves was obtained and analyzed by GC-MS. The antimicrobial activity of P. edulis leaf extract was determined by MIC test. XTT method was used to determine the antiproliferative activity. In the phytochemical analysis of P. edulis extract, dodecanoic acid, tetradecanoic acid, and n-hexadecanoic acid were found the most. The antimicrobial effect of P. edulis leaf extract was found against pathogenic microorganisms. In addition, P. edulis leaf extract was found to have high anticancer activity against OvCar and MCF-7 cell lines, while it had the highest effect on the PC-3 cell line. It is thought that the effectiveness of this antiproliferative and antimicrobial activity is related to the secondary metabolites determined by GC-MS analysis

Keywords

Passiflora edulis Dodecanoic acid Antimicrobial effect Secondary metabolite Antiproliferative effect

Thanks

The authors would like to thank Dr. Ceylan Hepokur for her aid and support and Dr. Hülya Özpınar for her aid in plant species identification.

References

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  • [2] Aguillón J., Arango S. S., Uribe D. F., Loango N., Cytotoxic and apoptotic activity of extracts from leaves and juice of Passiflora edulis, J. Liver. Res. Disord. Ther., 4(2) (2018) 67-71.
  • [3] Ramírez V., Arango S. S., Uribe D., Maldonado M. E., Aguillón J., Effect of the ethanolic extract of Passiflora edulis F. Flavicarpa leaves on viability, cytotoxicity and apoptosis of colon cancer cell lines, J. Chem. Pharm. Res., 9(6) (2017) 135-9.
  • [4] Antoni S., Soerjomataram I., Møller B., Bray F., Ferlay J., An assessment of GLOBOCAN methods for deriving national estimates of cancer incidence, Bulletin of the World Health Organization, 94(3) (2016) 174.
  • [5] Sari L. M., Sari D. K., Bustami A., Gazali A. D., Auerkari E. I., Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines, Journal of Pharmacy & Pharmacognosy Research, 12(4) (2024) 659-672.
  • [6] Çoban H. Ş., Çil N., Önder E., Abban Mete G., Anti-cancer effects of alpha lipoic acid, cisplatin and paclitaxel combination in the OVCAR-3 ovarian adenocarcinoma cell line, Molecular Biology Reports, 51(1) (2024) 485.
  • [7] Eisermann K., Tandon S., Bazarov A., Brett A., Fraizer G., Piontkivska H., Evolutionary conservation of zinc finger transcription factor binding sites in promoters of genes co-expressed with WT1 in prostate cancer, BMC Genomics, 9 (2008) 1-15.
  • [8] Choi E. S., Kim J. S., Kwon K. H., Kim H. S., Cho N. P., Cho S. D., Methanol extract of Sanguisorba officinalis L. with cytotoxic activity against PC3 human prostate cancer cells, Molecular Medicine Reports, 6(3) (2012) 670-674.
  • [9] Li H., Zhou P., Yang Q., Shen Y., Deng J., Li L., Zhao D., Comparative studies on anxiolytic activities and flavonoid compositions of Passiflora edulis ‘edulis’ and Passiflora edulis ‘flavicarpa’, Journal of Ethnopharmacology, 133(3) (2011) 1085-1090.
  • [10] Oliveira H. M., Espinheira M. J. C. L., de Souza F. M., Duarte S. F. P., David I. R., Sousa B. R., Evaluation of the antimicrobial potential of essential oil of the leaf of Passiflora edulis, Int. J. Adv. Eng. Res. Sci. (IJAERS), 6(5) (2019) 390-395.
  • [11] Johnson M., Maridass M., Irudayaraj V., Preliminary phytochemical and anti-bacterial studies on Passiflora edulis, Ethnobotanical Leaflets, (1) (2008) 51.
  • [12] Akanbi B. O., Bodunrin O. D., Olayanju S., Phytochemical screening and antibacterial activity of Passiflora edulis, Researcher, 3(5) (2011) 9-12.
  • [13] de Sousa G. F., Santos D. K. D. D. N., da Silva R. S., Barros B. R. D. S., da Cruz-Filho I. J., Ramos B. D. A., Lagos de Melo C. M., Evaluation of cytotoxic, immunomodulatory effects, antimicrobial activities and phytochemical constituents from various extracts of Passiflora edulis F. flavicarpa (Passifloraceae), Natural Product Research, 35(24) (2021) 5862-5866.
  • [14] Amaral R. G., Gomes S. V., Andrade L. N., Dos Santos S. A., Severino P., de Albuquerque Júnior R. L., Carvalho A. A., Cytotoxic, antitumor and toxicological profile of Passiflora alata leaf extract, Molecules, 25(20) (2020) 4814.
  • [15] Dias D. A., Urban S., Roessner U., A historical overview of natural products in drug discovery, Metabolites, 2(2) (2012) 303-336.
  • [16] Bohórquez-Moreno C. D., Öksüz K. E., Dinçer E., Hepokur C., Şen L., Plant-inspired adhesive and injectable natural hydrogels: in vitro and in vivo studies, Biotechnology Letters, 45(9) (2023) 1209–1222.
  • [17] Li C., Xin M., Li L., He X., Yi P., Tang Y., Li J., Zheng F., Liu G., Sheng J., Li Z., Sun J., Characterization of the aromatic profile of purple passion fruit (Passiflora edulis Sims) during ripening by HS-SPME-GC/MS and RNA sequencing, Food Chemistry, 355 (2021) 129685.
  • [18] Rizwana H., Al Otibi F., Al-Malki N., Chemical composition, FTIR studies and antibacterial activity of Passiflora edulis f. edulis (Fruit), J. Pure Appl. Microbiol., 13(4) (2019) 2489-2498.
  • [19] Hepokur C., Misir S., Tunç T., Tutar U., Hepokur A., Çiçek, M., In vitro antimicrobial, antioxidant, cytotoxic activities, and wound healing potential of Thymbra capitata ethanolic extract, Turkish Journal of Biochemistry, 45(6) (2020) 843-849.
  • [20] Gezegen H., Tutar U., Hepokur C., Ceylan M., Synthesis and biological evaluation of novel indenopyrazole derivatives, Journal of Biochemical and Molecular Toxicology, 33(5) (2019) e22285.
  • [21] Thomsen C., Rasmussen O., Lousen T., Holst J. J., Fenselau S., Schrezenmeir J., Hermansen K., Differential effects of saturated and monounsaturated fatty acids on postprandial lipemia and incretin responses in healthy subjects, The American Journal of Clinical Nutrition, 69 (6) (1999) 1135–1143.
  • [22] Calder P. C., Functional Roles of Fatty Acids and Their Effects on Human Health. JPEN, Journal of Parenteral and Enteral Nutrition, 39 (2015) (1S).
  • [23] Lalitharani S, Mohan VR, Regini GS., GC-MS analysis of ethanolic extract of Zanthoxylum rhetsa (roxb.) dc spines, J. Herbal Med. Toxicol. 4(1) (2010) 191-2.
  • [24] Ramaiya S. D., Bujang J. S., Zakaria M. H., Assessment of total phenolic, antioxidant, and antibacterial activities of Passiflora species, The Scientific World Journal, (1) (2014) 167309.
  • [25] Kannan S., Devi B. P., Jayakar B., Antibacterial evaluation of the methanolic extract of Passiflora edulis. Hygeia - Journal for Drugs and Medicine, 3(1) (2011) 46-49.
  • [26] Nugraha S. E., Achmad S., Sitompul E., Antibacterial activity of ethyl acetate fraction of passion fruit peel (Passiflora Edulis Sims) on Staphylococcus aureus and Escherichia coli, Indonesian Journal of Pharmaceutical and Clinical Research, 2(1) (2019) 07-12.
  • [27] Kuete V., Dzotam J. K., Voukeng I. K., Fankam A. G., Efferth T. Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines, Springerplus, 5 (2016) 1-12.
  • [28] Fotsing S. I., Pambe J. C. N., Silihe K. K., Yembeau N. L., Choupo A., Njamen D., Zingue S., Breast cancer cell growth arrest and chemopreventive effects of Passiflora edulis Sims (Passifloraceae) ethanolic leaves extract on a rat model of mammary carcinoma, Journal of Ethnopharmacology, 311 (2023) 116408.
  • [29] Amaral R. G., Gome, S. V. F., Luciano M. C. D. S., Pessoa C. D. Ó., Andrade L. N., Severino P., Carvalho A. A., Cytotoxic potential of 14 Passiflora species against cancer cells, Journal of Medicinal Plants Research, 13(7) (2019) 157-166.
  • [30] da Silva I. C., Kaluđerović G. N., de Oliveira P. F., Guimarães D. O., Quaresma C. H., Porzel A., Leal I. C., Apoptosis caused by triterpenes and phytosterols and antioxidant activity of an enriched flavonoid extract from Passiflora mucronata, Anti-Cancer Agents in Medicinal Chemistry, 18(10) (2018) 1405-1416.

Year 2024, Volume: 45 Issue: 3, 465 - 470, 30.09.2024

Tutku Tunç , Zeynep Sümer , Kerim Emre Öksüz

https://doi.org/10.17776/csj.1519699

Abstract

References

  • [1] Ripa F. A., Haque M., Nahar L., Islam M. M., Antibacterial, cytotoxic and antioxidant activity of Passiflora edulis Sims, Eur. J. Sci. Res., 31(4) (2009) 592-598.
  • [2] Aguillón J., Arango S. S., Uribe D. F., Loango N., Cytotoxic and apoptotic activity of extracts from leaves and juice of Passiflora edulis, J. Liver. Res. Disord. Ther., 4(2) (2018) 67-71.
  • [3] Ramírez V., Arango S. S., Uribe D., Maldonado M. E., Aguillón J., Effect of the ethanolic extract of Passiflora edulis F. Flavicarpa leaves on viability, cytotoxicity and apoptosis of colon cancer cell lines, J. Chem. Pharm. Res., 9(6) (2017) 135-9.
  • [4] Antoni S., Soerjomataram I., Møller B., Bray F., Ferlay J., An assessment of GLOBOCAN methods for deriving national estimates of cancer incidence, Bulletin of the World Health Organization, 94(3) (2016) 174.
  • [5] Sari L. M., Sari D. K., Bustami A., Gazali A. D., Auerkari E. I., Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines, Journal of Pharmacy & Pharmacognosy Research, 12(4) (2024) 659-672.
  • [6] Çoban H. Ş., Çil N., Önder E., Abban Mete G., Anti-cancer effects of alpha lipoic acid, cisplatin and paclitaxel combination in the OVCAR-3 ovarian adenocarcinoma cell line, Molecular Biology Reports, 51(1) (2024) 485.
  • [7] Eisermann K., Tandon S., Bazarov A., Brett A., Fraizer G., Piontkivska H., Evolutionary conservation of zinc finger transcription factor binding sites in promoters of genes co-expressed with WT1 in prostate cancer, BMC Genomics, 9 (2008) 1-15.
  • [8] Choi E. S., Kim J. S., Kwon K. H., Kim H. S., Cho N. P., Cho S. D., Methanol extract of Sanguisorba officinalis L. with cytotoxic activity against PC3 human prostate cancer cells, Molecular Medicine Reports, 6(3) (2012) 670-674.
  • [9] Li H., Zhou P., Yang Q., Shen Y., Deng J., Li L., Zhao D., Comparative studies on anxiolytic activities and flavonoid compositions of Passiflora edulis ‘edulis’ and Passiflora edulis ‘flavicarpa’, Journal of Ethnopharmacology, 133(3) (2011) 1085-1090.
  • [10] Oliveira H. M., Espinheira M. J. C. L., de Souza F. M., Duarte S. F. P., David I. R., Sousa B. R., Evaluation of the antimicrobial potential of essential oil of the leaf of Passiflora edulis, Int. J. Adv. Eng. Res. Sci. (IJAERS), 6(5) (2019) 390-395.
  • [11] Johnson M., Maridass M., Irudayaraj V., Preliminary phytochemical and anti-bacterial studies on Passiflora edulis, Ethnobotanical Leaflets, (1) (2008) 51.
  • [12] Akanbi B. O., Bodunrin O. D., Olayanju S., Phytochemical screening and antibacterial activity of Passiflora edulis, Researcher, 3(5) (2011) 9-12.
  • [13] de Sousa G. F., Santos D. K. D. D. N., da Silva R. S., Barros B. R. D. S., da Cruz-Filho I. J., Ramos B. D. A., Lagos de Melo C. M., Evaluation of cytotoxic, immunomodulatory effects, antimicrobial activities and phytochemical constituents from various extracts of Passiflora edulis F. flavicarpa (Passifloraceae), Natural Product Research, 35(24) (2021) 5862-5866.
  • [14] Amaral R. G., Gomes S. V., Andrade L. N., Dos Santos S. A., Severino P., de Albuquerque Júnior R. L., Carvalho A. A., Cytotoxic, antitumor and toxicological profile of Passiflora alata leaf extract, Molecules, 25(20) (2020) 4814.
  • [15] Dias D. A., Urban S., Roessner U., A historical overview of natural products in drug discovery, Metabolites, 2(2) (2012) 303-336.
  • [16] Bohórquez-Moreno C. D., Öksüz K. E., Dinçer E., Hepokur C., Şen L., Plant-inspired adhesive and injectable natural hydrogels: in vitro and in vivo studies, Biotechnology Letters, 45(9) (2023) 1209–1222.
  • [17] Li C., Xin M., Li L., He X., Yi P., Tang Y., Li J., Zheng F., Liu G., Sheng J., Li Z., Sun J., Characterization of the aromatic profile of purple passion fruit (Passiflora edulis Sims) during ripening by HS-SPME-GC/MS and RNA sequencing, Food Chemistry, 355 (2021) 129685.
  • [18] Rizwana H., Al Otibi F., Al-Malki N., Chemical composition, FTIR studies and antibacterial activity of Passiflora edulis f. edulis (Fruit), J. Pure Appl. Microbiol., 13(4) (2019) 2489-2498.
  • [19] Hepokur C., Misir S., Tunç T., Tutar U., Hepokur A., Çiçek, M., In vitro antimicrobial, antioxidant, cytotoxic activities, and wound healing potential of Thymbra capitata ethanolic extract, Turkish Journal of Biochemistry, 45(6) (2020) 843-849.
  • [20] Gezegen H., Tutar U., Hepokur C., Ceylan M., Synthesis and biological evaluation of novel indenopyrazole derivatives, Journal of Biochemical and Molecular Toxicology, 33(5) (2019) e22285.
  • [21] Thomsen C., Rasmussen O., Lousen T., Holst J. J., Fenselau S., Schrezenmeir J., Hermansen K., Differential effects of saturated and monounsaturated fatty acids on postprandial lipemia and incretin responses in healthy subjects, The American Journal of Clinical Nutrition, 69 (6) (1999) 1135–1143.
  • [22] Calder P. C., Functional Roles of Fatty Acids and Their Effects on Human Health. JPEN, Journal of Parenteral and Enteral Nutrition, 39 (2015) (1S).
  • [23] Lalitharani S, Mohan VR, Regini GS., GC-MS analysis of ethanolic extract of Zanthoxylum rhetsa (roxb.) dc spines, J. Herbal Med. Toxicol. 4(1) (2010) 191-2.
  • [24] Ramaiya S. D., Bujang J. S., Zakaria M. H., Assessment of total phenolic, antioxidant, and antibacterial activities of Passiflora species, The Scientific World Journal, (1) (2014) 167309.
  • [25] Kannan S., Devi B. P., Jayakar B., Antibacterial evaluation of the methanolic extract of Passiflora edulis. Hygeia - Journal for Drugs and Medicine, 3(1) (2011) 46-49.
  • [26] Nugraha S. E., Achmad S., Sitompul E., Antibacterial activity of ethyl acetate fraction of passion fruit peel (Passiflora Edulis Sims) on Staphylococcus aureus and Escherichia coli, Indonesian Journal of Pharmaceutical and Clinical Research, 2(1) (2019) 07-12.
  • [27] Kuete V., Dzotam J. K., Voukeng I. K., Fankam A. G., Efferth T. Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines, Springerplus, 5 (2016) 1-12.
  • [28] Fotsing S. I., Pambe J. C. N., Silihe K. K., Yembeau N. L., Choupo A., Njamen D., Zingue S., Breast cancer cell growth arrest and chemopreventive effects of Passiflora edulis Sims (Passifloraceae) ethanolic leaves extract on a rat model of mammary carcinoma, Journal of Ethnopharmacology, 311 (2023) 116408.
  • [29] Amaral R. G., Gome, S. V. F., Luciano M. C. D. S., Pessoa C. D. Ó., Andrade L. N., Severino P., Carvalho A. A., Cytotoxic potential of 14 Passiflora species against cancer cells, Journal of Medicinal Plants Research, 13(7) (2019) 157-166.
  • [30] da Silva I. C., Kaluđerović G. N., de Oliveira P. F., Guimarães D. O., Quaresma C. H., Porzel A., Leal I. C., Apoptosis caused by triterpenes and phytosterols and antioxidant activity of an enriched flavonoid extract from Passiflora mucronata, Anti-Cancer Agents in Medicinal Chemistry, 18(10) (2018) 1405-1416.
There are 30 citations in total.

Details

Primary Language English
Subjects Cell Development, Proliferation and Death, Bacteriology, Pharmaceutical Biochemistry
Journal Section Natural Sciences
Authors

Tutku Tunç 0000-0002-8274-9386

Zeynep Sümer 0000-0002-1520-3359

Kerim Emre Öksüz 0000-0001-7424-5930

Publication Date September 30, 2024
Submission Date July 20, 2024
Acceptance Date September 20, 2024
Published in Issue Year 2024Volume: 45 Issue: 3

Cite

APA Tunç, T., Sümer, Z., & Öksüz, K. E. (2024). Examination of Biological Activity of Passiflora edulis (Çarkıfelek) Extract via Phytochemical Analysis. Cumhuriyet Science Journal, 45(3), 465-470. https://doi.org/10.17776/csj.1519699
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