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Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp

Year 2022, Volume 43, Issue 3, 404 - 408, 30.09.2022
https://doi.org/10.17776/csj.1102028

Abstract

Candida species are commonly encountered strains associated with a wide range of infections. Unlike bacterial pathogens, fungal pathogens treatment is difficult and the development of resistance has been increasing at an alarming rate. In this study, the antifungal and antibiofilm effect of thyme oil, rosemary oil, mint oil, citronella oil, was tested on Candida albicans, Candida tropicalis, Candida kefyr, Candida glabrata, Candida parapsilosis isolated from clinical samples. The agar disc diffusion method was employed to determine the antifungal effect of the essential oils, and the inhibition of biofilm formation was assessed using microtiter biofilm inhibition assay. The results indicated that all the essential oils inhibited Candida strains and their biofilm in varying degrees. The highest antifungal activity in all isolates was observed in the thyme oil (>50mm), while rosemary oil showed the highest antibiofilm effect (>77%) in all tested strains. These findings led us to assume that the active components found in essential oils might be potential antifungal agents, adding to the repertoire of therapeutic options for the treatment of candidiasis. 

References

  • [1] Bongomin F., Gago S., Oladele R O., Denning DW., Global and multi-national prevalence of fungal diseases-estimate precision, Journal of Fungi, 3 (4) (2017) 57-69.
  • [2] Cleveland A., Harrison L., Farley M., Hollick R., Stein B., Chiller T., Declining Incidence of Candidemia and the Shifting Epidemiology of Candida Resistance in Two US Metropolitan Areas, 2008–2013: Results from Population-Based Surveillance., PLOS ONE, 10(3) (2015) e0120452..
  • [3] Nobile C., Johnson A., Candida albicans Biofilms and Human Disease, Annual Review Of Microbiology, 69(1) (2015) 71-92.
  • [4] Calogiuri G., Garvey L. H., Nettis E., Romita P., Di Leo E., Caruso R., Foti C., Skin allergy to azole antifungal agents for systemic use: A review of the literature, Recent Patents On Inflammation & Allergy Drug Discovery, 13 (2) (2019) 144–157.
  • [5] Di Bonaventura G., Spedicato I., Picciani C., D'Antonio D., Piccolomini R., In vitro pharmacodynamic characteristics of amphotericin B, caspofungin, fluconazole, and voriconazole against bloodstream isolates of infrequent Candida species from patients with hematologic malignancies, Antimicrobial Agents and Chemotherapy, 48(11) (2004) 4453–4456..
  • [6] Silva S., Rodrigues C., Araújo D., Rodrigues M., Henriques M., Candida Species Biofilms’ Antifungal Resistance, Journal Of Fungi, 3(1) (2017) 8-11
  • [7] Chandra J., Mukherjee, P., Candida Biofilms: Development, Architecture, and Resistance, Microbiology Spectrum, 3(4) (2015) 70-85.
  • [8] Nett J., Andes D., Contributions of the Biofilm Matrix to Candida Pathogenesis. Journal Of Fungi, 6(1) (2020) 21-33.
  • [9] Rajkowska K., Nowicka-Krawczyk P., Kunicka-Styczyńska A., Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules, 24(10) (2019) 42-54.
  • [10] Ali B., Al-Wabel N., Shams S., Ahamad A., Khan S., Anwar F., Essential oils used in aromatherapy: A systemic review, Asian Pacific Journal of Tropical Biomedicine, 5(8) (2015) 601-611.
  • [11] Bauer, A. W., W. M. M. Kirby, J. C. Sherris, and M. Turck. Antibiotic susceptibility testing by a standardized single disk method, American Journal of Clinical Pathology, 36 (1966) 493-496.
  • [12] Raveau R., Fontaine J., Lounès-Hadj Sahraoui A. Essential Oils as Potential Alternative Biocontrol Products against Plant Pathogens and Weeds: A Review, Foods, 9(3) (2020) 349-365.
  • [13] Onsare, J., and Arora, D. Antibiofilm potential of flavonoids extracted from Moringa oleifera seed coat against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, Journal of Applied Microbiology, 118 (3) (2015) 313–325.
  • [14] Jayathilaka E., Rajapaksha D., Nikapitiya C., De Zoysa M., Whang I., Antimicrobial and Anti-Biofilm Peptide Octominin for Controlling Multidrug-Resistant Acinetobacter baumannii, International Journal Of Molecular Sciences, 22(10) (2021) 53-55.
  • [15] Bachmann S., VandeWalle K., Ramage G., Patterson T., Wickes B., Graybill J., Lopez-Ribot J., In vitro activity of caspofungin against Candida albicans biofilms, Antimicrobial Agents and Chemotherapy, 46 (2002) 3591-3596
  • [16] Chandra J., Mukherjee P., Leidich S., Faddoul F., Hoyer L., Douglas J., Ghannoum M., Antifungal resistance of candidal biofilms formed on denture acrylic in vitro, Journal of Dental Research, 80(1) (2001) 903-908
  • [17] Shuford J., Piper K., Steckelberg J., PatelIn R., vitro biofilm characterization and activity of antifungal agents alone and in combination against sessile and planktonic clinical Candida albicans isolates, Diagnostic Microbiology and Infectious Disease, 57(3) (2007) 277-279
  • [18] Chouhan S, Sharma K., Guleria S., Antimicrobial activity of some essential oils -present status and future perspectives, Medicines, 4(3) (2017) 58-71.
  • [19] Bona E., Cantamessa S., Pavan M., Novello G., Massa N., Rocchetti, A.,Sensitivity of Candida albicans to essential oils: are they an alternative to antifungal agents?, Journal Of Applied Microbiology, 121(6) (2016) 1530-1545.
  • [20] Kızıl S., Hasımı N., Tolan V., Kılınc E. Yuksel U., Mineral content, essential oil components and biological activity of two Mentha species (M. piperita L., M. spicata L, Turkish Journal Of Field Crops, 15(2) (2010) 148-153.
  • [21] Saharkhiz M., Motamedi M., Zomorodian K., Pakshir K., Miri R., Hemyari, K., Chemical Composition, Antifungal and Antibiofilm Activities of the Essential Oil of Mentha piperita, ISRN Pharmaceutics, 20(12) (2012) 1-6.
  • [22] Benzaid C., Belmadani A., Djeribi R., Rouabhia M., The effects of Mentha piperita essential oil on C. albicans growth, transition, biofilm formation, and the expression of the secreted aspartyl proteinases genes, Antibiotics, 8(1) (2019) 10.
  • [23] Samber, N., Khan, A., Varma, A., & Manzoor, N. Synergistic anti-candidal activity and mode of action of Mentha piperita essential oil and its major components, Pharmaceutical Biology, 53(10) (2015)1496–1504.
  • [24] Sharma R., Rao R., Kumar S., Mahant S., Khatkar S., Therapeutic Potential of Citronella Essential Oil: A Review, Current Drug Discovery Technologies, 16(4) (2019) 330–339.
  • [25] Nakahara K., Alzoreky N.S., Yoshihashi T., Nguyen T., Trakoontivakorn G. Chemical composition and antifungal activity of essential oil from Cymbopogon nardus (Citronella Grass), Japan International Research Center for Agricultural Sciences, 37 (2013) 249–52.
  • [26] Khan M. S. A., Ahmad I., Antibiofilm activity of certain phytocompounds and their synergy with fluconazole against Candida albicans biofilms, Journal of Antimicrobial Chemotherapy, 67(3) (2011) 618–621.
  • [27] González-Trujano M., Peña E., Martínez A., Moreno J., Guevara-Fefer, P., Déciga-Campos, M., López-Muñoz F., Evaluation of the antinociceptive effect of Rosmarinus officinalis L. using three different experimental models in rodents, Journal of Ethnopharmacology, 11(3) (2007) 476–482.
  • [28] Trindade, L.A., Cordeiro, L.V., de Figuerêdo Silva, D. et al. The antifungal and antibiofilm activity of Cymbopogon nardus essential oil and citronellal on clinical strains of Candida albicans, Braz J. Microbiol., 53(3) 1231-1240.
  • [29] Matsuzaki, Y. , Tsujisawa, T. , Nishihara, T. , Nakamura, M. and Kakinoki, Y. Antifungal activity of chemotype essential oils from rosemary against Candida albicans, Open Journal of Stomatology, 3(2) (2013) 176-182.
  • [30] Alves, M., Gonçalves, M., Zuzarte, M., Alves-Silva, J., Cavaleiro, C., Cruz, M., & Salgueiro, L. Unveiling the Antifungal Potential of Two Iberian Thyme Essential Oils: Effect on C. albicans Germ Tube and Preformed Biofilms, Frontiers In Pharmacology, 10(2) (2019) 146-158
  • [31] Rajkowska, K., Nowicka-Krawczyk, P., & Kunicka-Styczyńska, A. Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules (Basel, Switzerland), 24(10) (2019) 154-165
  • [32] Meccatti V. M., Oliveira J. R. D., Figueira W., Lagareiro Netto A., Zamarioli,L. S., Marcucci, C., Camargo E., Carvalho A., Oliveira D., Rosmarinus officinalis L. (rosemary) extract has antibiofilm effect similar to the antifungal nystatin on Candida samples, Anais Da Academia Brasileira de Ciências, 93(2) (2021) e20190366.
  • [33] Ghasemi G., Alirezalu A., Ghosta Y., Jarrahi A., Safavi S. A., Abbas-Mohammadi M., Barba J., Munekata S., Domínguez R., Lorenzo M., Composition, Antifungal, Phytotoxic, and Insecticidal Activities of Thymus kotschyanus Essential Oil, Molecules, 25(5) (2020) 152-164.
  • [34] Nabavi M., Marchese A., Izadi M., Curti V., Daglia M., Nabavi F., Plants belonging to the genus Thymus as antibacterial agents: From farm to pharmaca, Food Chemistr, 173 (2015) 339–347.
  • [35] Rajkowska K., Nowicka-Krawczyk P., Kunicka-Styczyńska A., Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules, 24(10) (2019) 1954.

Year 2022, Volume 43, Issue 3, 404 - 408, 30.09.2022
https://doi.org/10.17776/csj.1102028

Abstract

References

  • [1] Bongomin F., Gago S., Oladele R O., Denning DW., Global and multi-national prevalence of fungal diseases-estimate precision, Journal of Fungi, 3 (4) (2017) 57-69.
  • [2] Cleveland A., Harrison L., Farley M., Hollick R., Stein B., Chiller T., Declining Incidence of Candidemia and the Shifting Epidemiology of Candida Resistance in Two US Metropolitan Areas, 2008–2013: Results from Population-Based Surveillance., PLOS ONE, 10(3) (2015) e0120452..
  • [3] Nobile C., Johnson A., Candida albicans Biofilms and Human Disease, Annual Review Of Microbiology, 69(1) (2015) 71-92.
  • [4] Calogiuri G., Garvey L. H., Nettis E., Romita P., Di Leo E., Caruso R., Foti C., Skin allergy to azole antifungal agents for systemic use: A review of the literature, Recent Patents On Inflammation & Allergy Drug Discovery, 13 (2) (2019) 144–157.
  • [5] Di Bonaventura G., Spedicato I., Picciani C., D'Antonio D., Piccolomini R., In vitro pharmacodynamic characteristics of amphotericin B, caspofungin, fluconazole, and voriconazole against bloodstream isolates of infrequent Candida species from patients with hematologic malignancies, Antimicrobial Agents and Chemotherapy, 48(11) (2004) 4453–4456..
  • [6] Silva S., Rodrigues C., Araújo D., Rodrigues M., Henriques M., Candida Species Biofilms’ Antifungal Resistance, Journal Of Fungi, 3(1) (2017) 8-11
  • [7] Chandra J., Mukherjee, P., Candida Biofilms: Development, Architecture, and Resistance, Microbiology Spectrum, 3(4) (2015) 70-85.
  • [8] Nett J., Andes D., Contributions of the Biofilm Matrix to Candida Pathogenesis. Journal Of Fungi, 6(1) (2020) 21-33.
  • [9] Rajkowska K., Nowicka-Krawczyk P., Kunicka-Styczyńska A., Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules, 24(10) (2019) 42-54.
  • [10] Ali B., Al-Wabel N., Shams S., Ahamad A., Khan S., Anwar F., Essential oils used in aromatherapy: A systemic review, Asian Pacific Journal of Tropical Biomedicine, 5(8) (2015) 601-611.
  • [11] Bauer, A. W., W. M. M. Kirby, J. C. Sherris, and M. Turck. Antibiotic susceptibility testing by a standardized single disk method, American Journal of Clinical Pathology, 36 (1966) 493-496.
  • [12] Raveau R., Fontaine J., Lounès-Hadj Sahraoui A. Essential Oils as Potential Alternative Biocontrol Products against Plant Pathogens and Weeds: A Review, Foods, 9(3) (2020) 349-365.
  • [13] Onsare, J., and Arora, D. Antibiofilm potential of flavonoids extracted from Moringa oleifera seed coat against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, Journal of Applied Microbiology, 118 (3) (2015) 313–325.
  • [14] Jayathilaka E., Rajapaksha D., Nikapitiya C., De Zoysa M., Whang I., Antimicrobial and Anti-Biofilm Peptide Octominin for Controlling Multidrug-Resistant Acinetobacter baumannii, International Journal Of Molecular Sciences, 22(10) (2021) 53-55.
  • [15] Bachmann S., VandeWalle K., Ramage G., Patterson T., Wickes B., Graybill J., Lopez-Ribot J., In vitro activity of caspofungin against Candida albicans biofilms, Antimicrobial Agents and Chemotherapy, 46 (2002) 3591-3596
  • [16] Chandra J., Mukherjee P., Leidich S., Faddoul F., Hoyer L., Douglas J., Ghannoum M., Antifungal resistance of candidal biofilms formed on denture acrylic in vitro, Journal of Dental Research, 80(1) (2001) 903-908
  • [17] Shuford J., Piper K., Steckelberg J., PatelIn R., vitro biofilm characterization and activity of antifungal agents alone and in combination against sessile and planktonic clinical Candida albicans isolates, Diagnostic Microbiology and Infectious Disease, 57(3) (2007) 277-279
  • [18] Chouhan S, Sharma K., Guleria S., Antimicrobial activity of some essential oils -present status and future perspectives, Medicines, 4(3) (2017) 58-71.
  • [19] Bona E., Cantamessa S., Pavan M., Novello G., Massa N., Rocchetti, A.,Sensitivity of Candida albicans to essential oils: are they an alternative to antifungal agents?, Journal Of Applied Microbiology, 121(6) (2016) 1530-1545.
  • [20] Kızıl S., Hasımı N., Tolan V., Kılınc E. Yuksel U., Mineral content, essential oil components and biological activity of two Mentha species (M. piperita L., M. spicata L, Turkish Journal Of Field Crops, 15(2) (2010) 148-153.
  • [21] Saharkhiz M., Motamedi M., Zomorodian K., Pakshir K., Miri R., Hemyari, K., Chemical Composition, Antifungal and Antibiofilm Activities of the Essential Oil of Mentha piperita, ISRN Pharmaceutics, 20(12) (2012) 1-6.
  • [22] Benzaid C., Belmadani A., Djeribi R., Rouabhia M., The effects of Mentha piperita essential oil on C. albicans growth, transition, biofilm formation, and the expression of the secreted aspartyl proteinases genes, Antibiotics, 8(1) (2019) 10.
  • [23] Samber, N., Khan, A., Varma, A., & Manzoor, N. Synergistic anti-candidal activity and mode of action of Mentha piperita essential oil and its major components, Pharmaceutical Biology, 53(10) (2015)1496–1504.
  • [24] Sharma R., Rao R., Kumar S., Mahant S., Khatkar S., Therapeutic Potential of Citronella Essential Oil: A Review, Current Drug Discovery Technologies, 16(4) (2019) 330–339.
  • [25] Nakahara K., Alzoreky N.S., Yoshihashi T., Nguyen T., Trakoontivakorn G. Chemical composition and antifungal activity of essential oil from Cymbopogon nardus (Citronella Grass), Japan International Research Center for Agricultural Sciences, 37 (2013) 249–52.
  • [26] Khan M. S. A., Ahmad I., Antibiofilm activity of certain phytocompounds and their synergy with fluconazole against Candida albicans biofilms, Journal of Antimicrobial Chemotherapy, 67(3) (2011) 618–621.
  • [27] González-Trujano M., Peña E., Martínez A., Moreno J., Guevara-Fefer, P., Déciga-Campos, M., López-Muñoz F., Evaluation of the antinociceptive effect of Rosmarinus officinalis L. using three different experimental models in rodents, Journal of Ethnopharmacology, 11(3) (2007) 476–482.
  • [28] Trindade, L.A., Cordeiro, L.V., de Figuerêdo Silva, D. et al. The antifungal and antibiofilm activity of Cymbopogon nardus essential oil and citronellal on clinical strains of Candida albicans, Braz J. Microbiol., 53(3) 1231-1240.
  • [29] Matsuzaki, Y. , Tsujisawa, T. , Nishihara, T. , Nakamura, M. and Kakinoki, Y. Antifungal activity of chemotype essential oils from rosemary against Candida albicans, Open Journal of Stomatology, 3(2) (2013) 176-182.
  • [30] Alves, M., Gonçalves, M., Zuzarte, M., Alves-Silva, J., Cavaleiro, C., Cruz, M., & Salgueiro, L. Unveiling the Antifungal Potential of Two Iberian Thyme Essential Oils: Effect on C. albicans Germ Tube and Preformed Biofilms, Frontiers In Pharmacology, 10(2) (2019) 146-158
  • [31] Rajkowska, K., Nowicka-Krawczyk, P., & Kunicka-Styczyńska, A. Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules (Basel, Switzerland), 24(10) (2019) 154-165
  • [32] Meccatti V. M., Oliveira J. R. D., Figueira W., Lagareiro Netto A., Zamarioli,L. S., Marcucci, C., Camargo E., Carvalho A., Oliveira D., Rosmarinus officinalis L. (rosemary) extract has antibiofilm effect similar to the antifungal nystatin on Candida samples, Anais Da Academia Brasileira de Ciências, 93(2) (2021) e20190366.
  • [33] Ghasemi G., Alirezalu A., Ghosta Y., Jarrahi A., Safavi S. A., Abbas-Mohammadi M., Barba J., Munekata S., Domínguez R., Lorenzo M., Composition, Antifungal, Phytotoxic, and Insecticidal Activities of Thymus kotschyanus Essential Oil, Molecules, 25(5) (2020) 152-164.
  • [34] Nabavi M., Marchese A., Izadi M., Curti V., Daglia M., Nabavi F., Plants belonging to the genus Thymus as antibacterial agents: From farm to pharmaca, Food Chemistr, 173 (2015) 339–347.
  • [35] Rajkowska K., Nowicka-Krawczyk P., Kunicka-Styczyńska A., Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules, 24(10) (2019) 1954.

Details

Primary Language English
Subjects Pharmacology and Pharmacy, Biology
Journal Section Natural Sciences
Authors

Bydaa ATRON> (Primary Author)
SİVAS CUMHURİYET ÜNİVERSİTESİ
0000-0002-7571-9277
Türkiye


Hanaou AHAMADA>
SIVAS CUMHURIYET UNIVERSITY
0000-0002-0000-2239
Türkiye


Ayşe Hümeyra TAŞKIN KAFA>
SIVAS CUMHURIYET UNIVERSITY
0000-0002-7282-4928
Türkiye


Cem ÇELİK>
SIVAS CUMHURIYET UNIVERSITY
0000-0002-7141-5874
Türkiye


Mürşit HASBEK>
SIVAS CUMHURIYET UNIVERSITY
0000-0002-5217-8607
Türkiye

Publication Date September 30, 2022
Application Date April 12, 2022
Acceptance Date August 4, 2022
Published in Issue Year 2022, Volume 43, Issue 3

Cite

APA Atron, B. , Ahamada, H. , Taşkın Kafa, A. H. , Çelik, C. & Hasbek, M. (2022). Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp . Cumhuriyet Science Journal , 43 (3) , 404-408 . DOI: 10.17776/csj.1102028