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Year 2021, Volume: 42 Issue: 3, 536 - 544, 24.09.2021

Michael Osho

https://doi.org/10.17776/csj.835765

Abstract

References

  • [1] Duhan J.S., Kumar A., Tanwar S.K., Bio-ethanol production from starchy part of tuberous plant (potato) using Saccharomyces cerevisiae MTCC-170, African Journal of Microbiology Research, 7(46) (2013) 5253-5260.
  • [2] Anuj K.C., Ravinder R., Lakshmi M.N., Rao V., Ravindra P., Economic and environmental impact of bio- ethanol production technology, Biotechnology and Molecular Biology Review, 2(1) (2007) 14-32.
  • [3] Brook A.A., Ethanol potential of local yeast strains isolated from ripe banana peels, African Journal of Biotechnology, 7(20) (2008) 3749 – 3752.
  • [4] Naylor R.L., Liska A., Burke M., Falcon W.P., Gaskell J., Razello S., Cassaman K., The effect: Biofuel, food security and the environment, Environmental Microbiology, 49(9) (2007) 30-43.
  • [5] Aisien F.A., Aguye M.D., Aisien E.T., Blending of ethanol produced from cassava waste water with gasoline as source of automobile fuel, Electronic Journal of Environment, Agriculture and Food Chemistry, 9(5) (2010) 946-950.
  • [6] Klass D.L., Biomass for renewable energy fuels and chemicals, London: Academic Press, (1998) 544.
  • [7] Limatainen H., Kuokkanen T., Kaariainen J., Development of bio-ethanol production from waste potatoes. In: Pongracz, E., (ed.) Proceedings of the Waste Minimization and Resources Use Optimization Conference, Finland, Oulu: Oulu University Press, (2004) 123-129.
  • [8] Adarsha R., Asha D.L. Balaji R.R., Production of bio-ethanol from Pectobacterium carotovorum induced soft rotten potatoes, African Journal of Microbiology Research, 4(12) (2010) 1340-1342.
  • [9] Srinorakutara T., Kaewvimol L., Saengow I., Approach of cassava waste water pre-treatment for fuel ethanol production in Thailand, Journal of Science Research, 31(1) (2008) 77-84.
  • [10] Muhamud F. Bin I., Production of Bio-ethanol from Tapioca Starch using Saccharomyces cerevisiae, PD thesis, University of Malaysia Pahang, (2009).
  • [11] Lee W., Jin Y., Evaluation of ethanol production activity by engineered Saccharomyces cerevisiae fermenting cellobiose through the phosphorolytic pathway in simultaneous saccharification and fermentation of cellulose, Journal of Microbiology and Biotechnology, 27(9) (2017) 1649–1656.
  • [12] Abouzied M.M., Reddy C.A., Direct fermentation of potato starch to ethanol by co-culture of Aspergillus niger and Saccharomyces cerevisiae, Applied and Environmental Microbiology, 52(5) (1986) 1055-1059.
  • [13] Oyeleke S.B., Dauda B., Oyewole O.A., Okoliegbe I.N., Ojebode T., Production of Bio-ethanol from cassava and sweet potato peels, Advances in Environmental Biology, 6(1) (2012) 241-245.
  • [14] George P., Aggelos G., Aikaterini K., Styliani K., Dimitris K., Diomi M., Bioethanol Production from Food Waste Applying the Multienzyme System Produced On-Site by Fusarium oxysporum F3 and Mixed Microbial Cultures, Fermentation, 6 (2020) 39.
  • [15] Farias D.F., Carvalho A.F.U., Oliveira C.C., Sousa N.M., Rocha-Bezerrra L.C.B., Ferreira P.M.P., Hissa D.C., Alternative method for quantification of alpha-amylase activity, Brazilians Journal of Biology, 70(2) (2010) 405-407.
  • [16] Sreedevi S., Reddy B.B.N., Isolation, screening and optimization of phytase production from newly isolated Bacillus sp. C43, International Journal of Pharmacy and Biological Science, 2(2) (2012) 218-231.
  • [17] Kumar S., Stecher G., Tamura K., MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets, Molecular Biology Evolution, 33(7) (2016) 1870-1874.
  • [18] Amadi P.U., Ifeanacho M.O., Impact of changes in fermentation time, volume of yeast, and mass of plantain pseudo-stem substrate on the simultaneous saccharification and fermentation potentials of African land snail digestive juice and yeast, Journal of Genetic Engineering and Biotechnology, 14(2) (2016) 289-297.
  • [19] Akponah E., Akpomie O.O., Analysis of the suitability of yam, potato and cassava root peels for bioethanol production using Saccharomyces cerevisae, International Research Journal of Microbiology, 2(10) (2011) 393-398.
  • [20] Swain M.R., Mishra J., Thatoi H., Bio-ethanol Production from Sweet Potato (Ipomoea batatas L.) Flour using Co-Culture of Trichoderma sp. and Saccharomyces cerevisiae in Solid-State Fermentation, Brazil Archive of Biology Technology, 56 (2) (2013) 171-179.
  • [21] Amadi B.A., Agomuo E.N., Ibegbulan CO. Research Methods in Biochemistry, Owerri-Nigeria: Supreme Publishers, (2004); 93-99.
  • [22] Caputi A,. Ueda M., Brown T., American Journal of Enology and Viticulture, 19 (1968) 160–165.
  • [23] Yoswathana N., Phuriphipat P., Bioethanol Production from Rice Straw, Energy Research Journal, 11 (2010) 26–31.
  • [24] Hennessy R.C., Jørgensen N.O.G., Scavenius C., Enghild J.J., Greve-Poulsen M., Sørensen O.B., Stougaard P.A., Screening Method for the Isolation of Bacteria Capable of Degrading Toxic Steroidal Glycoalkaloids Present in Potato, Frontier Microbiology, 9 (2018) 2648.
  • [25] Gudeta D., Isolation and characterization of starch degrading rhizobacteria from soil of Jimma University Main Campus, Ethiopia, African Journal of Microbiology Research, 12(32) (2018) 788-795.
  • [26] Rijal N., Starch Hydrolysis Test: Principle, Procedure, Results. In Mucormycosis: Pathogenesis, Clinical Manifestations and Treatment. Available at: https://microbeonline.com/starch-hydrolysis-test/. Retrieved September, 2021.
  • [27] Zakpa H.D., Mak-Mensah E.E., Johnson F.S., Production of ethanol from corncobs using Aspergillus niger and Saccharomyces cerevisiae in simultaneous saccharification and fermentation, African Journal of Biotechnology, 8(13) (2009) 3018-3022.
  • [28] Bekele A., Fite A., Alemu S., Sewhunegn T., Bogele E., Simachew M., Debele T., Production of bio-ethanol from waste potato peel collected from University of Gondar, student’s cafeteria, Global Journal of Biochemistry and Biotechnology, 3(3) (2015) 132-140.
  • [29] Rabinkov A., Miron T., Konstantinovski L., Wilchek M., Mirelman D., Weiner L., The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins, Biochemical and Biophysical Acta., 1379 (1998) 233–244.
  • [30] Teixeira M.C., Godinho C.P., Cabrito T.R., Mira N.P., Sa´Correia I., Increased expression of the yeast multidrug resistance ABC transporter Pdr18 leads to increased ethanol tolerance and ethanol production in high gravity alcoholic fermentation, Microbial Cell Facts, 11(1) (2012) 98.
  • [31] Kishimoto J., Ehama R., Wu L., Jiang S., Jiang N., Burgeson R.E., Re-Selective activation of versican promoter by epithelial-mesenchymal interactions during hair follicle development, Proceedings of National Academy of Science (USA), 96 (1999) 7336-7341.
  • [32] Martins N., Petropoulos S., Ferreira C.F.R., Chemical composition and bioactive compounds of garlic (Allium sativum L.) as affected by pre- and post-harvest conditions: A review, Food Chemistry, 211 (2016) 41-50.
  • [33] Igbokwe P.K., Idogwu C.N., Nwabanne J.T., Enzymatic Hydrolysis and Fermentation of Plantain Peels: Optimization and Kinetic Studies, Advances in Chemical Engineering and Science, 6 (2016) 216-235.
  • [34] Joshi J., Dhungana P., Prajapati B., Maharjan R., Poudyal P., Yadav M., Mainali M., Yadav A.P., Bhattarai T., Sreerama L., Enhancement of Ethanol Production in Electrochemical Cell by Saccharomyces cerevisiae (CDBT2) and Wicker hamomycesanomalus (CDBT7), Frontier Energy Research, 7 (2019) 70.
  • [35] Rita H.R.B., Mariana S.T.A., Luísa S.S., Ana MRBX. Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption, Energies, 13(3) (2020) 744.
  • [36] Olofssen K., Bertilsson M., Liden G., A short review on SSF – an interesting process option for ethanol production from lignocellulose feedstocks, Biotechnology for Biofuels, 1(7) (2008) 1-14.
  • [37] Arotupin D.J., Evaluation of microorganisms from cassava waste for production of amylase and cellulase, Resource Journal of Microbiology, 2(5) (2007) 475-480.
  • [38] Zhang P., Hai H., Sun D., Yuan W., Liu W., Ding R., Teng M., Ma L., Tian J., Chen C., A high throughput method for total alcohol determination in fermentation broths, BMC Biotechnology, 19 (2019) 30.

Enzymatic degradation and fermentation of Corn Bran for Bioethanol production by Pseudomonas aeruginosa AU4738 and Saccharomyces cerevisiae using Co-culture technique

Year 2021, Volume: 42 Issue: 3, 536 - 544, 24.09.2021

Michael Osho

https://doi.org/10.17776/csj.835765

Abstract

Ethanol is one of the bioenergy sources with low environmental and high efficiency impact. The aim of this study was to screen for the bacterial isolate capable of degrading starch, investigate the enzymatic hydrolysis and fermentation of corn bran through submerged fermentation using co-culture technique for bioethanol production. The isolate was identified using 16S rRNA sequence technique as Pseudomonas aeruginosa AU4738. Corn bran was used as substrates with and without garlic powder (Allium sativum L.) as activator and subsequently optimized for production of bioethanol. Reducing sugar from the hydrolysate and ethanol concentration of the distillate were analyzed using spectrophotometry and gas chromatography mass spectrometry techniques respectively. There was an increase in glucose concentration (23.8% and 17.8%) in the culture medium with and without activator at 48 h respectively but steadily decreased from 72 h to 168 h. Maximum ethanol concentration obtained in substrate culture with activator was 35% higher compared with that without activator at 120 h fermentation time. Thus a cheap, renewable and readily available agricultural waste has been effectively utilized as substrate for bioethanol production and incorporation of activator also had significant effect on the viability of fermenting organisms thus subjugating the intolerance of alcohol concentration.

Keywords

Corn bran Bioethanol Pseudomonas aeruginosa AU4738 Saccharomyces cerevisiae Co-culture Technique

References

  • [1] Duhan J.S., Kumar A., Tanwar S.K., Bio-ethanol production from starchy part of tuberous plant (potato) using Saccharomyces cerevisiae MTCC-170, African Journal of Microbiology Research, 7(46) (2013) 5253-5260.
  • [2] Anuj K.C., Ravinder R., Lakshmi M.N., Rao V., Ravindra P., Economic and environmental impact of bio- ethanol production technology, Biotechnology and Molecular Biology Review, 2(1) (2007) 14-32.
  • [3] Brook A.A., Ethanol potential of local yeast strains isolated from ripe banana peels, African Journal of Biotechnology, 7(20) (2008) 3749 – 3752.
  • [4] Naylor R.L., Liska A., Burke M., Falcon W.P., Gaskell J., Razello S., Cassaman K., The effect: Biofuel, food security and the environment, Environmental Microbiology, 49(9) (2007) 30-43.
  • [5] Aisien F.A., Aguye M.D., Aisien E.T., Blending of ethanol produced from cassava waste water with gasoline as source of automobile fuel, Electronic Journal of Environment, Agriculture and Food Chemistry, 9(5) (2010) 946-950.
  • [6] Klass D.L., Biomass for renewable energy fuels and chemicals, London: Academic Press, (1998) 544.
  • [7] Limatainen H., Kuokkanen T., Kaariainen J., Development of bio-ethanol production from waste potatoes. In: Pongracz, E., (ed.) Proceedings of the Waste Minimization and Resources Use Optimization Conference, Finland, Oulu: Oulu University Press, (2004) 123-129.
  • [8] Adarsha R., Asha D.L. Balaji R.R., Production of bio-ethanol from Pectobacterium carotovorum induced soft rotten potatoes, African Journal of Microbiology Research, 4(12) (2010) 1340-1342.
  • [9] Srinorakutara T., Kaewvimol L., Saengow I., Approach of cassava waste water pre-treatment for fuel ethanol production in Thailand, Journal of Science Research, 31(1) (2008) 77-84.
  • [10] Muhamud F. Bin I., Production of Bio-ethanol from Tapioca Starch using Saccharomyces cerevisiae, PD thesis, University of Malaysia Pahang, (2009).
  • [11] Lee W., Jin Y., Evaluation of ethanol production activity by engineered Saccharomyces cerevisiae fermenting cellobiose through the phosphorolytic pathway in simultaneous saccharification and fermentation of cellulose, Journal of Microbiology and Biotechnology, 27(9) (2017) 1649–1656.
  • [12] Abouzied M.M., Reddy C.A., Direct fermentation of potato starch to ethanol by co-culture of Aspergillus niger and Saccharomyces cerevisiae, Applied and Environmental Microbiology, 52(5) (1986) 1055-1059.
  • [13] Oyeleke S.B., Dauda B., Oyewole O.A., Okoliegbe I.N., Ojebode T., Production of Bio-ethanol from cassava and sweet potato peels, Advances in Environmental Biology, 6(1) (2012) 241-245.
  • [14] George P., Aggelos G., Aikaterini K., Styliani K., Dimitris K., Diomi M., Bioethanol Production from Food Waste Applying the Multienzyme System Produced On-Site by Fusarium oxysporum F3 and Mixed Microbial Cultures, Fermentation, 6 (2020) 39.
  • [15] Farias D.F., Carvalho A.F.U., Oliveira C.C., Sousa N.M., Rocha-Bezerrra L.C.B., Ferreira P.M.P., Hissa D.C., Alternative method for quantification of alpha-amylase activity, Brazilians Journal of Biology, 70(2) (2010) 405-407.
  • [16] Sreedevi S., Reddy B.B.N., Isolation, screening and optimization of phytase production from newly isolated Bacillus sp. C43, International Journal of Pharmacy and Biological Science, 2(2) (2012) 218-231.
  • [17] Kumar S., Stecher G., Tamura K., MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets, Molecular Biology Evolution, 33(7) (2016) 1870-1874.
  • [18] Amadi P.U., Ifeanacho M.O., Impact of changes in fermentation time, volume of yeast, and mass of plantain pseudo-stem substrate on the simultaneous saccharification and fermentation potentials of African land snail digestive juice and yeast, Journal of Genetic Engineering and Biotechnology, 14(2) (2016) 289-297.
  • [19] Akponah E., Akpomie O.O., Analysis of the suitability of yam, potato and cassava root peels for bioethanol production using Saccharomyces cerevisae, International Research Journal of Microbiology, 2(10) (2011) 393-398.
  • [20] Swain M.R., Mishra J., Thatoi H., Bio-ethanol Production from Sweet Potato (Ipomoea batatas L.) Flour using Co-Culture of Trichoderma sp. and Saccharomyces cerevisiae in Solid-State Fermentation, Brazil Archive of Biology Technology, 56 (2) (2013) 171-179.
  • [21] Amadi B.A., Agomuo E.N., Ibegbulan CO. Research Methods in Biochemistry, Owerri-Nigeria: Supreme Publishers, (2004); 93-99.
  • [22] Caputi A,. Ueda M., Brown T., American Journal of Enology and Viticulture, 19 (1968) 160–165.
  • [23] Yoswathana N., Phuriphipat P., Bioethanol Production from Rice Straw, Energy Research Journal, 11 (2010) 26–31.
  • [24] Hennessy R.C., Jørgensen N.O.G., Scavenius C., Enghild J.J., Greve-Poulsen M., Sørensen O.B., Stougaard P.A., Screening Method for the Isolation of Bacteria Capable of Degrading Toxic Steroidal Glycoalkaloids Present in Potato, Frontier Microbiology, 9 (2018) 2648.
  • [25] Gudeta D., Isolation and characterization of starch degrading rhizobacteria from soil of Jimma University Main Campus, Ethiopia, African Journal of Microbiology Research, 12(32) (2018) 788-795.
  • [26] Rijal N., Starch Hydrolysis Test: Principle, Procedure, Results. In Mucormycosis: Pathogenesis, Clinical Manifestations and Treatment. Available at: https://microbeonline.com/starch-hydrolysis-test/. Retrieved September, 2021.
  • [27] Zakpa H.D., Mak-Mensah E.E., Johnson F.S., Production of ethanol from corncobs using Aspergillus niger and Saccharomyces cerevisiae in simultaneous saccharification and fermentation, African Journal of Biotechnology, 8(13) (2009) 3018-3022.
  • [28] Bekele A., Fite A., Alemu S., Sewhunegn T., Bogele E., Simachew M., Debele T., Production of bio-ethanol from waste potato peel collected from University of Gondar, student’s cafeteria, Global Journal of Biochemistry and Biotechnology, 3(3) (2015) 132-140.
  • [29] Rabinkov A., Miron T., Konstantinovski L., Wilchek M., Mirelman D., Weiner L., The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins, Biochemical and Biophysical Acta., 1379 (1998) 233–244.
  • [30] Teixeira M.C., Godinho C.P., Cabrito T.R., Mira N.P., Sa´Correia I., Increased expression of the yeast multidrug resistance ABC transporter Pdr18 leads to increased ethanol tolerance and ethanol production in high gravity alcoholic fermentation, Microbial Cell Facts, 11(1) (2012) 98.
  • [31] Kishimoto J., Ehama R., Wu L., Jiang S., Jiang N., Burgeson R.E., Re-Selective activation of versican promoter by epithelial-mesenchymal interactions during hair follicle development, Proceedings of National Academy of Science (USA), 96 (1999) 7336-7341.
  • [32] Martins N., Petropoulos S., Ferreira C.F.R., Chemical composition and bioactive compounds of garlic (Allium sativum L.) as affected by pre- and post-harvest conditions: A review, Food Chemistry, 211 (2016) 41-50.
  • [33] Igbokwe P.K., Idogwu C.N., Nwabanne J.T., Enzymatic Hydrolysis and Fermentation of Plantain Peels: Optimization and Kinetic Studies, Advances in Chemical Engineering and Science, 6 (2016) 216-235.
  • [34] Joshi J., Dhungana P., Prajapati B., Maharjan R., Poudyal P., Yadav M., Mainali M., Yadav A.P., Bhattarai T., Sreerama L., Enhancement of Ethanol Production in Electrochemical Cell by Saccharomyces cerevisiae (CDBT2) and Wicker hamomycesanomalus (CDBT7), Frontier Energy Research, 7 (2019) 70.
  • [35] Rita H.R.B., Mariana S.T.A., Luísa S.S., Ana MRBX. Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption, Energies, 13(3) (2020) 744.
  • [36] Olofssen K., Bertilsson M., Liden G., A short review on SSF – an interesting process option for ethanol production from lignocellulose feedstocks, Biotechnology for Biofuels, 1(7) (2008) 1-14.
  • [37] Arotupin D.J., Evaluation of microorganisms from cassava waste for production of amylase and cellulase, Resource Journal of Microbiology, 2(5) (2007) 475-480.
  • [38] Zhang P., Hai H., Sun D., Yuan W., Liu W., Ding R., Teng M., Ma L., Tian J., Chen C., A high throughput method for total alcohol determination in fermentation broths, BMC Biotechnology, 19 (2019) 30.
There are 38 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Natural Sciences
Authors

Michael Osho 0000-0003-1177-8363

Publication Date September 24, 2021
Submission Date December 4, 2020
Acceptance Date September 7, 2021
Published in Issue Year 2021Volume: 42 Issue: 3

Cite

APA Osho, M. (2021). Enzymatic degradation and fermentation of Corn Bran for Bioethanol production by Pseudomonas aeruginosa AU4738 and Saccharomyces cerevisiae using Co-culture technique. Cumhuriyet Science Journal, 42(3), 536-544. https://doi.org/10.17776/csj.835765
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