New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme

Birnur Akkaya; Recep Akkaya

doi:10.17776/csj.423415

EN TR

New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme

Abstract

In this study, we investigated adsorption behaviour of lysozyme on poly(hydroxyethylmethacrylate-expanded perlite) [P(Hema-Ep)]. For this purpose, we synthesized P(Hema-Ep) composite by bulk polymerization and it was characterized by SEM, FTIR and swelling ratio. The fresh chicken egg white lysozyme was used as a model protein, had an isoelectric point (pI) 10.7 and molecular weight (MW) 14 kDa. Adsorption capacity was found 51 mg g^-1 at room temperature and in 0.1 M pH=8.0 phosphate buffer. Experiments on desorption and reusability were also performed. It appears that P(Hema-Ep) composites can be used ten times without lost of efficiency. Lysozyme purification, without causing any denaturation, can be performed from chicken egg white by using this new synthesized composite in a single step.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Birnur Akkaya ^*
Türkiye

Recep Akkaya
Türkiye

Publication Date

September 30, 2018

Submission Date

May 14, 2018

Acceptance Date

July 5, 2018

Published in Issue

Year 1970 Volume: 39 Number: 3

DOI

https://doi.org/10.17776/csj.423415

IZ

https://izlik.org/JA89SC93AG

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RIS / Bibtex

APA

Akkaya, B., & Akkaya, R. (2018). New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme. Cumhuriyet Science Journal, 39(3), 694-702. https://doi.org/10.17776/csj.423415

Cited By

Chitosan–poly(acrylamide-co-maleic acid) composite synthesis, characterization, and investigation of protein adsorption behavior

Polymer Bulletin

https://doi.org/10.1007/s00289-022-04259-2

New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme

New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme

Abstract

Keywords

Poli(Hidroksietilmetakrilat-Genişletilmiş Perlit) Kompoziti ile Yumurta Akından Lizozimin Tek Basamakta Saflaştırılması

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Chitosan–poly(acrylamide-co-maleic acid) composite synthesis, characterization, and investigation of protein adsorption behavior