Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı

Mert Akınlı; Volkan Tekin; Fazilet Müftüler; Gökçen Topal; Yasemin Parlak; Gül Gümüşer; Uğur Avcıbaşı

doi:10.15671/hjbc.656021

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Year 2021, , 243 - 252, 17.05.2021

Mert Akınlı Volkan Tekin Fazilet Müftüler Gökçen Topal Yasemin Parlak Gül Gümüşer Uğur Avcıbaşı

https://doi.org/10.15671/hjbc.656021

Abstract

References

REFERENCES
1. J.J. Knapik, R.A. Steelman, S.S. Hoedebecke, Prevalence of dietary supplement use by athletes: Systematic review and meta-analysis, Sports. Med., 46 (2016) 103–123.
2. R.J. Maughan, P.L. Greenhaff, P. Hespel, Dietary supplements for athletes: Emerging trends and recurring themes, J. Sport. Sci., 29 (2011) 57–66.
3. D.T. Thomas, K.A. Erdman, L.M. Burke, Position of the academy of nutrition and dietetics, dietitians of Canada, and the american college of sports Medicine: Nutrition and athletic performance, J. Acad. of Nutr. Diet., 116 (2016) 501–528.
4. L.M. Burke, L. Cato, Supplements and sports foods. In L. M. Burke, & V. Deakin (Eds.). Clinical sports nutrition 5th ed., 493–591, McGraw- Hill Pty Ltd, Australia, 2015.
5. A. Sánchez-Oliver, M. Miranda-León, E. Guerra-Hernández, Statistical analysis of the consumption of nutritional and dietary supplements in gyms [Estudioestadístico del consumo de suplementos nutricionales y dietéticos en gimnasios], Arch. Latin de Nutri., 58 (2008) 221–227.
6. A. Sánchez-Oliver, M.T. Miranda León, E. Guerra-Hernández, Prevalence of protein supplement use at gyms [Estudio estadístico del consumo de suplementos proteícos en gimnasios], Nutr. Hosp., 26 (2011) 1168–1174.
7. R.L. Walzem, C.J. Dillard, J.B. German, Whey components: Millennia of evolution create functionalities for mammalian nutrition: What we know and what we may be overlooking, Crit. Rev. Food Sci. Nutr., 42 (2001) 353 375.
8. G.W. Smithers, Whey and whey proteins-from “gutter to gold”, Int. Dairy J., 18 (2008) 695 704.
9. H.M. Farrell, R. Jimenez Flores, G.T. Bleck, Nomenclature of the proteins of cows' milk sixth revision, J. Dairy Sci., 87 (2004) 1641 1674.
10. C. Mollea, L. Marmo, F. Bosco F, Valorisation of cheese whey, a by product from the dairy industry. In: Food Industry, Muzzalupo I (ed). InTech., 12 (2013) 549-588.
11. K. Marshall, Therapeutic applications of whey protein, Altern. Med. Rev., 9 (2004) 136 156.
12. A.J. Sánchez-Oliver, J. Contreras-Calderónc, J.M. Puya-Brazad, Quality analysis of commercial protein powder supplements and relation to characteristics declared by manufacturer, LWT - Food Sci. Tech., 97 (2018) 100–108.
13. F. Gür, M. Güzel, N. Öncül N, Biological and Physiological Activities of Whey Proteins and Their Derivatives, Akademik Gıda 8 (2010) 23-31.
14. A.O. Afuwape, M.W. Turner, S, Strobel. Oral administration of bovine whey proteins to mice elicits opposing immunoregulatory responses and is adjuvant dependent, Clin. Exp. Immunol., 136 (2004) 40–48.
15. A.M. Holwerda, I.W.K. Kouw, J. Trommelen, Physical Activity Performed in the Evening Increases the Overnight Muscle Protein Synthetic Response to Presleep Protein Ingestion in Older Men, J. Nutr., 146 (2016) 1307–1314.
16. E. Kerasioti, D. Stagos, A.M. Tsatsakis, Effects of sheep/goat whey protein dietary supplementationon the redox status of rats, Mol. Med. Rep., 17 (2018) 5774-5781.
17. C. Karagözlü, M. Bayarer M, The Functional Properties of Whey Proteins and Their Health Effects, Ege Üniv. Ziraat Fak. Derg., 41 (2004) 197-207.
18. X. Zhen, X. Wang, C. Yang, Synthesis, Cellular Uptake, and Biodistribution of Whey-Rich Nanoparticles, Macromol. Biosci., 14 (2014) 1149–1159.
19. B. Noh, T. Richardson, Incorporation of Radiolabeled Whey Proteins into Casein Micelles by Heat Processing, J. Dairy Sci., 72 (1989) 1724-1731.
20. L.D.R. Davis, W.J. Spencer, V.T. Pham, 14C radiolabeling of proteins to monitor biodistribution of ingested proteins, Anal. Biochem. 410 (2011) 57–61.
21. A. Kanda, K. Nakayama, C. Sanbongi, Effects of Whey, Caseinate, or Milk Protein Ingestion on Muscle Protein Synthesis after Exercise, Nutrients 8 (2016) 339-343.
22. G. Rehner, M. Heil, M. Auge, Effect of Proteins on availability of zinc I. Gastrointestinal transit time of casein and whey protein and zinc absorption in weaned rats, Z. Ernahrungswiss 24 (1985) 245-255.
23. K. Schwochau, Technetium Chemistry and Radiopharmaceutical Applications, J. Dairy Sci., 1 (2000) 460-468.
24. D.D. Dischino, J.M. Welch, R.M. Kilbourn, Relationship between Lipophilicity and Brain Extraction of C-11-Radiopharmaceuticals, J. Nucl. Med., 24 (1983) 1030–1038.
25. S. Chattopadhyay, M.K. Das, R. Vanaja R, Purification and stabilization of 99mTc-d, l-HMPAO: role of organic extractants, Nucl. Med. Biol., 28 (2001) 741–744.
26. A.Y. Kilcar, F.Z.B. Muftuler, H. Enginar, Synthesis, characterization and biodistribution of 99mTc-Bioquin-HMPAO (99mTc-BH) as a novel brain imaging agent, J. Radioanal. Nucl. Chem., 302 (2014) 563–573.
27. M. Liu, Y. Zheng, U. Avcibasi, Novel 99mTc(III)-azide complexes [99mTc(N3)(CDO)(CDOH)2B-R] (CDOH2 = cyclohexanedione dioxime) as potential radiotracers for heart imaging, Nucl. Med. Biol., 43 (2016) 732–741.
28. Ö. Uyaroğlu, H. Demiroğlu, G. Topal, Radiosynthesis and biodistribution of 99mTc-Sulfamethoxazole: a novel molecule for in-vivo infection imaging, Med. Chem. Res., 26 (2017) 3149–3157.
29. H. Demiroğlu, G. Topal, Y. Parlak, Radiosynthesis and Biodistribution of 99mTc-Trimethoprim: A Novel Radiolabeled Antibiotic for Bacterial Infection Imaging Using Experimental Animals, Kafkas Univ. Vet. Fak. Derg., 24 (2018) 393-400.

Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı

Year 2021, , 243 - 252, 17.05.2021

Mert Akınlı Volkan Tekin Fazilet Müftüler Gökçen Topal Yasemin Parlak Gül Gümüşer Uğur Avcıbaşı

https://doi.org/10.15671/hjbc.656021

Abstract

Bu çalışmanın esas amacı Whey proteinin nükleer tıpta bir görüntüleme ajanı olarak kullanılıp kullanılamayacağını tespit etmektir. Bunun için; Whey proteini kalay klorür indirgenme metodu kullanılarak Tc-99m ile işaretlenmiştir. Radyokromatografi çalışmalarında kağıt elektroforezi tekniği kullanılmıştır. Uygun hareketli faz olarak SF (serum fizyolojik) kullanılmıştır. Aynı zamanda Tc-99m ile işaretli Whey protein’in bağlanma verimi yaklaşık %95 olarak bulunmuştur. Daha sonra, sırasıyla lipofilite ve stabilite çalışmaları gerçekleştirilmiştir. Son olarak Albino Wistar sıçanlarda görüntüleme ve biyodağılım çalışmaları yapılmıştır.
Sonuç olarak, bütün elde edilen sonuçlar Tc-99m ile işaretli Whey protein’in gelecekte meme, yumurtalık ve pankreas kanserlerinin teşhisinde kullanılabileceğini göstermiştir.

Keywords

Whey Proteini, Biyodağılım, 99mTc, Sintigrafi, Protein Tozu Suplemanı

References

REFERENCES
1. J.J. Knapik, R.A. Steelman, S.S. Hoedebecke, Prevalence of dietary supplement use by athletes: Systematic review and meta-analysis, Sports. Med., 46 (2016) 103–123.
2. R.J. Maughan, P.L. Greenhaff, P. Hespel, Dietary supplements for athletes: Emerging trends and recurring themes, J. Sport. Sci., 29 (2011) 57–66.
3. D.T. Thomas, K.A. Erdman, L.M. Burke, Position of the academy of nutrition and dietetics, dietitians of Canada, and the american college of sports Medicine: Nutrition and athletic performance, J. Acad. of Nutr. Diet., 116 (2016) 501–528.
4. L.M. Burke, L. Cato, Supplements and sports foods. In L. M. Burke, & V. Deakin (Eds.). Clinical sports nutrition 5th ed., 493–591, McGraw- Hill Pty Ltd, Australia, 2015.
5. A. Sánchez-Oliver, M. Miranda-León, E. Guerra-Hernández, Statistical analysis of the consumption of nutritional and dietary supplements in gyms [Estudioestadístico del consumo de suplementos nutricionales y dietéticos en gimnasios], Arch. Latin de Nutri., 58 (2008) 221–227.
6. A. Sánchez-Oliver, M.T. Miranda León, E. Guerra-Hernández, Prevalence of protein supplement use at gyms [Estudio estadístico del consumo de suplementos proteícos en gimnasios], Nutr. Hosp., 26 (2011) 1168–1174.
7. R.L. Walzem, C.J. Dillard, J.B. German, Whey components: Millennia of evolution create functionalities for mammalian nutrition: What we know and what we may be overlooking, Crit. Rev. Food Sci. Nutr., 42 (2001) 353 375.
8. G.W. Smithers, Whey and whey proteins-from “gutter to gold”, Int. Dairy J., 18 (2008) 695 704.
9. H.M. Farrell, R. Jimenez Flores, G.T. Bleck, Nomenclature of the proteins of cows' milk sixth revision, J. Dairy Sci., 87 (2004) 1641 1674.
10. C. Mollea, L. Marmo, F. Bosco F, Valorisation of cheese whey, a by product from the dairy industry. In: Food Industry, Muzzalupo I (ed). InTech., 12 (2013) 549-588.
11. K. Marshall, Therapeutic applications of whey protein, Altern. Med. Rev., 9 (2004) 136 156.
12. A.J. Sánchez-Oliver, J. Contreras-Calderónc, J.M. Puya-Brazad, Quality analysis of commercial protein powder supplements and relation to characteristics declared by manufacturer, LWT - Food Sci. Tech., 97 (2018) 100–108.
13. F. Gür, M. Güzel, N. Öncül N, Biological and Physiological Activities of Whey Proteins and Their Derivatives, Akademik Gıda 8 (2010) 23-31.
14. A.O. Afuwape, M.W. Turner, S, Strobel. Oral administration of bovine whey proteins to mice elicits opposing immunoregulatory responses and is adjuvant dependent, Clin. Exp. Immunol., 136 (2004) 40–48.
15. A.M. Holwerda, I.W.K. Kouw, J. Trommelen, Physical Activity Performed in the Evening Increases the Overnight Muscle Protein Synthetic Response to Presleep Protein Ingestion in Older Men, J. Nutr., 146 (2016) 1307–1314.
16. E. Kerasioti, D. Stagos, A.M. Tsatsakis, Effects of sheep/goat whey protein dietary supplementationon the redox status of rats, Mol. Med. Rep., 17 (2018) 5774-5781.
17. C. Karagözlü, M. Bayarer M, The Functional Properties of Whey Proteins and Their Health Effects, Ege Üniv. Ziraat Fak. Derg., 41 (2004) 197-207.
18. X. Zhen, X. Wang, C. Yang, Synthesis, Cellular Uptake, and Biodistribution of Whey-Rich Nanoparticles, Macromol. Biosci., 14 (2014) 1149–1159.
19. B. Noh, T. Richardson, Incorporation of Radiolabeled Whey Proteins into Casein Micelles by Heat Processing, J. Dairy Sci., 72 (1989) 1724-1731.
20. L.D.R. Davis, W.J. Spencer, V.T. Pham, 14C radiolabeling of proteins to monitor biodistribution of ingested proteins, Anal. Biochem. 410 (2011) 57–61.
21. A. Kanda, K. Nakayama, C. Sanbongi, Effects of Whey, Caseinate, or Milk Protein Ingestion on Muscle Protein Synthesis after Exercise, Nutrients 8 (2016) 339-343.
22. G. Rehner, M. Heil, M. Auge, Effect of Proteins on availability of zinc I. Gastrointestinal transit time of casein and whey protein and zinc absorption in weaned rats, Z. Ernahrungswiss 24 (1985) 245-255.
23. K. Schwochau, Technetium Chemistry and Radiopharmaceutical Applications, J. Dairy Sci., 1 (2000) 460-468.
24. D.D. Dischino, J.M. Welch, R.M. Kilbourn, Relationship between Lipophilicity and Brain Extraction of C-11-Radiopharmaceuticals, J. Nucl. Med., 24 (1983) 1030–1038.
25. S. Chattopadhyay, M.K. Das, R. Vanaja R, Purification and stabilization of 99mTc-d, l-HMPAO: role of organic extractants, Nucl. Med. Biol., 28 (2001) 741–744.
26. A.Y. Kilcar, F.Z.B. Muftuler, H. Enginar, Synthesis, characterization and biodistribution of 99mTc-Bioquin-HMPAO (99mTc-BH) as a novel brain imaging agent, J. Radioanal. Nucl. Chem., 302 (2014) 563–573.
27. M. Liu, Y. Zheng, U. Avcibasi, Novel 99mTc(III)-azide complexes [99mTc(N3)(CDO)(CDOH)2B-R] (CDOH2 = cyclohexanedione dioxime) as potential radiotracers for heart imaging, Nucl. Med. Biol., 43 (2016) 732–741.
28. Ö. Uyaroğlu, H. Demiroğlu, G. Topal, Radiosynthesis and biodistribution of 99mTc-Sulfamethoxazole: a novel molecule for in-vivo infection imaging, Med. Chem. Res., 26 (2017) 3149–3157.
29. H. Demiroğlu, G. Topal, Y. Parlak, Radiosynthesis and Biodistribution of 99mTc-Trimethoprim: A Novel Radiolabeled Antibiotic for Bacterial Infection Imaging Using Experimental Animals, Kafkas Univ. Vet. Fak. Derg., 24 (2018) 393-400.

There are 30 citations in total.

Details

Primary Language	English
Subjects	Engineering
Journal Section	Research Article
Authors	Mert Akınlı 0000-0003-0653-9261 Volkan Tekin 0000-0002-0264-3814 Fazilet Müftüler 0000-0001-8184-9660 Gökçen Topal 0000-0002-2268-8921 Yasemin Parlak 0000-0002-3682-7611 Gül Gümüşer 0000-0001-7103-2323 Uğur Avcıbaşı 0000-0002-3718-4195
Publication Date	May 17, 2021
Acceptance Date	November 9, 2020
Published in Issue	Year 2021

Cite

APA	Akınlı, M., Tekin, V., Müftüler, F., Topal, G., et al. (2021). Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı. Hacettepe Journal of Biology and Chemistry, 49(3), 243-252. https://doi.org/10.15671/hjbc.656021
AMA	Akınlı M, Tekin V, Müftüler F, Topal G, Parlak Y, Gümüşer G, Avcıbaşı U. Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı. HJBC. May 2021;49(3):243-252. doi:10.15671/hjbc.656021
Chicago	Akınlı, Mert, Volkan Tekin, Fazilet Müftüler, Gökçen Topal, Yasemin Parlak, Gül Gümüşer, and Uğur Avcıbaşı. “Tc-99m Ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı”. Hacettepe Journal of Biology and Chemistry 49, no. 3 (May 2021): 243-52. https://doi.org/10.15671/hjbc.656021.
EndNote	Akınlı M, Tekin V, Müftüler F, Topal G, Parlak Y, Gümüşer G, Avcıbaşı U (May 1, 2021) Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı. Hacettepe Journal of Biology and Chemistry 49 3 243–252.
IEEE	M. Akınlı, “Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı”, HJBC, vol. 49, no. 3, pp. 243–252, 2021, doi: 10.15671/hjbc.656021.
ISNAD	Akınlı, Mert et al. “Tc-99m Ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı”. Hacettepe Journal of Biology and Chemistry 49/3 (May 2021), 243-252. https://doi.org/10.15671/hjbc.656021.
JAMA	Akınlı M, Tekin V, Müftüler F, Topal G, Parlak Y, Gümüşer G, Avcıbaşı U. Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı. HJBC. 2021;49:243–252.
MLA	Akınlı, Mert et al. “Tc-99m Ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı”. Hacettepe Journal of Biology and Chemistry, vol. 49, no. 3, 2021, pp. 243-52, doi:10.15671/hjbc.656021.
Vancouver	Akınlı M, Tekin V, Müftüler F, Topal G, Parlak Y, Gümüşer G, Avcıbaşı U. Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı. HJBC. 2021;49(3):243-52.

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