KRONİK STRESİN ADOLESAN SIÇANLARDA NÖTROFİL LENFOSİT ORANI VE ERİTROSİT DAĞILIM GENİŞLİĞİNE OLAN ETKİSİNİN DEĞERLENDİRİLMESİ
Abstract
Giriş: Kronik stres maruziyeti homestaz durumunu bozmakta ve immun değişikliklere yol açmaktadır. Eritrosit dağılım genişliği (RDW) ve nötrofil lenfosit oranı (NLR), sistemik inflamasyonu değerlendirmede kullanılabilmektedir. Bu çalışmada kronik stres modelinin, adolesan dönem sıçanlarda hematolojik parametreleri nasıl etkilediği incelenmiştir.
Yöntemler: 16 adet adolesan Wistar albino erkek sıçan, kontrol grubu ve stres grubu olarak iki gruba ayrılmıştır. Stres grubuna modifiye edilmiş kronik aralıklı soğuk stres modeli 28 gün boyunca uygulanmıştır. Hematolojik parametreler ölçülmüştür. Anlamlılık düzeyi t-testi ile ölçülmüştür.
Bulgular: Kronik stres grubunda eritrosit dağılım genişliği değeri, kontrol grubuna kıyasla anlamlı olarak artmış bulunmuştur (p<0,05). Nötrofil lenfosit oranı düzeyleri açısından kronik stres grubu ve kontrol grubu arasında anlamlı fark bulunmamıştır (p>0.05). Hemoglobin düzeyleri, lökosit sayısı ve lökosit alt grupları dağılımı açısından iki grup arasında anlamlı fark gözlenmemiştir (p>0,05).
Sonuç: Eritrosit dağılım genişliği artışı, stresin etkilerinin değerlendirilmesinde kullanılabilir. Nötrofil lenfosit oranının stresle ilişkisinin değerlendirilmesi için daha fazla çalışmaya ihtiyaç vardır. Ek olarak, uygulanan stres protokolünün çeşidi ve maruziyet süresi sonuçları etkileyebilmektedir.
Keywords
eritrosit dağılım genişliği kronik stres nötrofil lenfosit oranı red cell distribution width chronic stress neutrophil lymphocyte ratio
Supporting Institution
Ankara Üniversitesi Bilimsel Araştırma Projeleri Müdürlüğü
Project Number
21L0230005
References
- 1. Bali A, Jaggi AS. Preclinical experimental stress studies: protocols, assessment and comparison. Eur J Pharmacol 2015;746:282-92.
- 2. Xu B, Lian S, Guo JR, et al. Activation of the MAPK signaling pathway induces upregulation of pro-apoptotic proteins in the hippocampi of cold stressed adolescent mice. Neurosci Lett 2019;699:97-102.
- 3. Broom DM, Fraser AF. Domestic animal behaviour and welfare. 5th ed. Wallingford, CABI Publishing; 2015.
- 4. Zahorec R. Neutrophil-to-lymphocyte ratio, past, present and future perspectives. Bratisl Lek Listy 2021;122:474-88.
- 5. Song M, Graubard BI, Rabkin CS, Engels EA. Neutrophil-to-lymphocyte ratio and mortality in the United States general population. Sci Rep 2021;11:464-73.
- 6. Hickman DL. Evaluation of the neutrophil:lymphocyte ratio as an indicator of chronic distress in the laboratory mouse. Lab Anim (NY) 2017;46:303-7.
- 7. Swan MP, Hickman DL. Evaluation of the neutrophil-lymphocyte ratio as a measure of distress in rats. Lab Anim (NY) 2014;43:276-82.
- 8. Davis AK, Maney DL, Maerz JC. The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology 2008;22:760-72.
- 9. Karagülle M. Covid-19 Hastalarında D-dimer ve Eritrosit Dağılım Genişliği Arasındaki İlişkinin Klinik Önemi. Eskisehir Medical Journal 2022;3:123-9.
- 10. Lippi G, Turcato G, Cervellin G, Sanchis-Gomar F. Red blood cell distribution width in heart failure: A narrative review. World J Cardiol 2018;10:6-14.
- 11. Demircan F, Gozel N, Kilinc F, Ulu R, Atmaca M. The Impact of Red Blood Cell Distribution Width and Neutrophil/Lymphocyte Ratio on the Diagnosis of Major Depressive Disorder. Neurol Ther 2016;5:27-33.
- 12. Ige AO, Iwaloye OI, Adewoye EO. Metformin Effects are Augmented by Chronic Intermittent Cold Stress in High Fat Diet Fed Male Wistar Rats. Niger J Physiol Sci 2017;32:47-54.
- 13. Edwards E, King JA, Fray JC. Increased basal activity of the HPA axis and renin-angiotensin system in congenital learned helpless rats exposed to stress early in development. Int J Dev Neurosci 1999;17:805-12.
- 14. Gray JD, Rubin TG, Hunter RG, McEwen BS. Hippocampal gene expression changes underlying stress sensitization and recovery. Mol Psychiatry 2014;19:1171-8.
- 15. El Marzouki H, Aboussaleh Y, Najimi M, Chigr F, Ahami A. Effect of Cold Stress on Neurobehavioral and Physiological Parameters in Rats. Front Physiol 2021;12:1-20.
- 16. Kalay N, Aytekin M, Kaya MG, et al. The relationship between inflammation and slow coronary flow: increased red cell distribution width and serum uric acid levels. Turk Kardiyol Dern Ars 2011;39:463-8.
- 17. Zamanian Z, Yousefinejad S, Dehghan SF, Rahmani A. Hemato-biochemical responses of rats co-exposed to heat stress and trichloroethylene vapors. Russian Open Medical Journal 2021;10:405-14.
- 18. Peng Y-F, Zhong S-M, Luo B, Qin Y-h, Wei Y. Evaluation of red blood cell distribution width and neutrophil to lymphocyte ratio in patients with major depressive disorder. Neurol Ther 2016;5:27-33.
- 19. Jacob Filho W, Lima CC, Paunksnis MRR, et al. Reference database of hematological parameters for growing and aging rats. Aging Male 2018;21:145-8.
- 20. Nemzek JA, Bolgos GL, Williams BA, Remick DG. Differences in normal values for murine white blood cell counts and other hematological parameters based on sampling site. Inflamm Res 2001;50:523-7.
- 21. Northern AL, Rutter SM, Peterson CM. Cyclic changes in the concentrations of peripheral blood immune cells during the normal menstrual cycle. Proc Soc Exp Biol Med 1994;207:81-8.
EVALUATION OF THE EFFECT OF CHRONIC STRESS ON NEUTROPHIL LYMPHOCYTE RATIO AND RED CELL DISTRIBUTION WIDTH IN ADOLESCENT RATS
Abstract
Introduction: Chronic stres exposure impairs homestasis and causes immune changes. Red cell distribution width (RDW) and neutrophil lymphocyte ratio (NLR) can be used to evaluate systemic inflammation. In this study, we investigated the effects of chronic stress on hematological parameters in adolescent rats.
Methods: 16 adolescent Wistar albino male rats were divided into 2 groups as control group and chronic stres group. Modified chronic intermittent cold stress model was applied to the chronic stres group for 28 days. Hematological parameters were measured. The significance level between groups was assessed by Student’s 𝑡-test.
Results: The red cell distribution width value was found to be significantly increased in the chronic stres group compared to the control group. (p<0.05) There was no difference between the chronic stres group and the control group in terms of neutrophil lymphocyte ratio levels (p>0.05). There was no significant difference between the two groups in terms of hemoglobin levels, leukocyte count and distribution of leukocyte subgroups (p>0.05).
Conclusion: The increase in red cell distribution width can be used to evaluate the effects of stress. More studies are needed to evaluate the relationship between neutrophil lymphocyte ratio and the effect of stress. In addition, the type of stres protocol and the exposure time may affect the results.
Project Number
21L0230005
References
- 1. Bali A, Jaggi AS. Preclinical experimental stress studies: protocols, assessment and comparison. Eur J Pharmacol 2015;746:282-92.
- 2. Xu B, Lian S, Guo JR, et al. Activation of the MAPK signaling pathway induces upregulation of pro-apoptotic proteins in the hippocampi of cold stressed adolescent mice. Neurosci Lett 2019;699:97-102.
- 3. Broom DM, Fraser AF. Domestic animal behaviour and welfare. 5th ed. Wallingford, CABI Publishing; 2015.
- 4. Zahorec R. Neutrophil-to-lymphocyte ratio, past, present and future perspectives. Bratisl Lek Listy 2021;122:474-88.
- 5. Song M, Graubard BI, Rabkin CS, Engels EA. Neutrophil-to-lymphocyte ratio and mortality in the United States general population. Sci Rep 2021;11:464-73.
- 6. Hickman DL. Evaluation of the neutrophil:lymphocyte ratio as an indicator of chronic distress in the laboratory mouse. Lab Anim (NY) 2017;46:303-7.
- 7. Swan MP, Hickman DL. Evaluation of the neutrophil-lymphocyte ratio as a measure of distress in rats. Lab Anim (NY) 2014;43:276-82.
- 8. Davis AK, Maney DL, Maerz JC. The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology 2008;22:760-72.
- 9. Karagülle M. Covid-19 Hastalarında D-dimer ve Eritrosit Dağılım Genişliği Arasındaki İlişkinin Klinik Önemi. Eskisehir Medical Journal 2022;3:123-9.
- 10. Lippi G, Turcato G, Cervellin G, Sanchis-Gomar F. Red blood cell distribution width in heart failure: A narrative review. World J Cardiol 2018;10:6-14.
- 11. Demircan F, Gozel N, Kilinc F, Ulu R, Atmaca M. The Impact of Red Blood Cell Distribution Width and Neutrophil/Lymphocyte Ratio on the Diagnosis of Major Depressive Disorder. Neurol Ther 2016;5:27-33.
- 12. Ige AO, Iwaloye OI, Adewoye EO. Metformin Effects are Augmented by Chronic Intermittent Cold Stress in High Fat Diet Fed Male Wistar Rats. Niger J Physiol Sci 2017;32:47-54.
- 13. Edwards E, King JA, Fray JC. Increased basal activity of the HPA axis and renin-angiotensin system in congenital learned helpless rats exposed to stress early in development. Int J Dev Neurosci 1999;17:805-12.
- 14. Gray JD, Rubin TG, Hunter RG, McEwen BS. Hippocampal gene expression changes underlying stress sensitization and recovery. Mol Psychiatry 2014;19:1171-8.
- 15. El Marzouki H, Aboussaleh Y, Najimi M, Chigr F, Ahami A. Effect of Cold Stress on Neurobehavioral and Physiological Parameters in Rats. Front Physiol 2021;12:1-20.
- 16. Kalay N, Aytekin M, Kaya MG, et al. The relationship between inflammation and slow coronary flow: increased red cell distribution width and serum uric acid levels. Turk Kardiyol Dern Ars 2011;39:463-8.
- 17. Zamanian Z, Yousefinejad S, Dehghan SF, Rahmani A. Hemato-biochemical responses of rats co-exposed to heat stress and trichloroethylene vapors. Russian Open Medical Journal 2021;10:405-14.
- 18. Peng Y-F, Zhong S-M, Luo B, Qin Y-h, Wei Y. Evaluation of red blood cell distribution width and neutrophil to lymphocyte ratio in patients with major depressive disorder. Neurol Ther 2016;5:27-33.
- 19. Jacob Filho W, Lima CC, Paunksnis MRR, et al. Reference database of hematological parameters for growing and aging rats. Aging Male 2018;21:145-8.
- 20. Nemzek JA, Bolgos GL, Williams BA, Remick DG. Differences in normal values for murine white blood cell counts and other hematological parameters based on sampling site. Inflamm Res 2001;50:523-7.
- 21. Northern AL, Rutter SM, Peterson CM. Cyclic changes in the concentrations of peripheral blood immune cells during the normal menstrual cycle. Proc Soc Exp Biol Med 1994;207:81-8.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Articles |
| Authors | |
| Project Number | 21L0230005 |
| Early Pub Date | July 28, 2023 |
| Publication Date | July 28, 2023 |
| Published in Issue | Year 2023 Volume: 4 Issue: 2 |
Cite
