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Karate Zeon Pestisitinin Mutajenik Aktivitesinin Ames Testi ile Değerlendirilmesi

Year 2022, Volume: 22 Issue: 3, 465 - 469, 30.06.2022

Arzu Özkara

https://doi.org/10.35414/akufemubid.1069842

Abstract

Pestisitler, ürünlerin kalitesi ve veriminin artmasında tarımda yaygın olarak kullanılırlar. Ancak pestisitler, hedef olmayan organizmalar üzerinde pestisit direnci, mutajenik ve karsinojenik etkilerinden dolayı çevre için büyük bir endişe kaynağı haline gelmiştir. Bu çalışmada, sentetik bir piretroid insektisit olan Karate Zeon'un potansiyel mutajenitesinin araştırılması amaçlanmıştır. Salmonella typhimurium mutajenite testi (Ames testi), S9 fraksiyonunun varlığı ve yokluğunda Karate Zeon insektisitinin iki suş (TA98 ve TA100) üzerindeki mutajenitesini değerlendirmek için kullanılmıştır. Bu çalışmada, Karate zeonun 250, 25, 2.5, 0.25, 0.025 µg/plak dozları kullanılmıştır. Karate Zeon pestisiti, hem TA98 hem de TA100 üzerinde metabolik aktivasyon varlığı ya da yokluğunda 250 μg/plak konsantrasyonunda mutajenik aktivite göstermiştir.

Keywords

Ames Testi Karate zeon Mutajenite Pestisit

References

  • Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological profile for pyrethrins and pyrethroids. Atlanta, GA: Department of Health and Human Services, Public Health Service, Toxicology (2003).
  • Ahouangninou, C., Martin, T., Edorh, P., Bio-Bangana, S., Samuel, O., St-Laurent, L. and Fayomi, B., 2012. Characterization of health and environmental risks of pesticide use in market-gardening in the rural city of Tori-Bossito in Benin, West Africa. Journal of Environmental Protection, 3, 241-248.
  • Ames, B.N., McCann, J., Yamasaki, E., 1975. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutation Research, 31, 347-364.
  • Asita, A.O. and Mokhobo, M.M., 2013. Clastogenic and cytotoxic effects of four pesticides used to control insect pests of stored products on root meristems of Allium cepa. Environment and Natural Resources Journal, 3(2), 133-145.
  • Basri, H., Topaktaş, M., Rencüzoğulları, E., Kayraldız, A., Dönbak, L., Dağlıoğlu, Y.K., 2008. Genotoxic potential of cyfluthrin. Mutation Research, 1(2), 49-50.
  • Casida, J., Quistad, G., 1998. Golden age of insecticide research: past, present, or future? Annual Review of Entomology, 43, 1-16.
  • Grossman, N., 2007. Influence of pyrethroids and piperonylbutoxide on histamine release from isolated rat mast cells. Journal of Inflammation Research, 56, 473-478.
  • Jensen, H.K., Konradsen, F., Dalsgaard, A., 2011. Pesticide use and self-reported symptoms of acute pesticide poisoning among aquatic farmers in Phnom Penh, Cambodia. International Journal of Toxicology, 639814.
  • Leme, D.M. and Marin-Morales, M.A., 2009. Allium cepa test in environmental monitoring: A review on its application. Mutation Research, 682, 71-81.
  • Maron, D.M., Ames, B.N., 1983. Revised methods for the Salmonella mutagenicity test. Mutation Research, 113, 173-215.
  • Medina, D., Prieto, A., Ettiene, G., Buscema, I. and Abreu de, V.A., 1999. Persistence of Organophosphorus Pesticide Residues in Limón River Waters. Bulletin of Environmental Contamination and Toxicology, 63(1), 39-44.
  • Miadokova, E., Vickova, V., Duhova, V., Trebaticka, M., Garajova, K., Grolmus, J., Podstavkova, S., Vicek, D., 1992. Effects of supercypermethrin, a synthetic developmental pyrethroid, on four biological test systems. Mutation Research, 3, 161-168.
  • Ministry of the Environment Compilation of pollutant release and transfer register data Ministry of the Environment; in Japanese, 2011.
  • Osman, K.A., Al-Humaid, A.M., Al-Rehiayani, S.M. and Al- Redhaiman, K.N., 2010. Monitoring of pesticide residues in vegetables marketed in Al-Qassim region, Saudi Arabia. Ecotoxicology and Environmental Safety, 73(6), 1433-1439.
  • Oulhote, Y., Bouchard, M.F., 2013. Urinary metabolites of organophosphate and pyrethroid pesticides and behavioral problems in Canadian children. Environmental Health Perspectives, 121, 1378-1384.
  • Özkara, A., 2017. Evaluation of Siperkor Pesticide Mutagenicity with Ames Test. Afyon Kocatepe University Journal of Sciences and Engineering, 17, 393-398.
  • Özkara, A., 2019. Assessment of cytotoxicity and mutagenicity of insecticide Demond EC25 in Allium cepa and Ames Test. Caryologia, 72(2), 21-27.
  • Pimentel, D., Greiner, A. and Bashore, T., 1998. Economic and environmental costs of pesticides use, Environmental Toxicology, Current Developments, London UK, Gordon and Breach Science Publisher, 121-187.
  • Pluijmen, M., Drevon, C., Montesano, R., Malaveille, C., Hautefeuille, A., Bartsch, H., 1984. Lack of mutagenicity of synthetic pyrethroids in Salmonella typhimurium strains and in V79, Chinese hamster ovary cells. Mutation Research, 137, 7-15.
  • Saillenfait, A.M., Ndiaye, D., Sabaté, J.P., 2015. Pyrethroids: exposure and health effects an update. International Journal of Hygiene and Environmental Health, 218(3), 281-92.
  • Shukla, Y., Taneja, P., 2000. Mutagenic evaluation of deltamethrin using rodent dominant lethal assay. Mutation Research, 467, 119-127.
  • Song, Y., Kai, J., Song, X., Zhang, W., Li, L., 2015. Long-term toxic effects of deltamethrin and fenvalerante in soil. Journal of Hazardous Materials, 289, 158-164.
  • Surralles, J., Xamena, N., Creus, A., Catalan, J., Norppa, H., Marcos, R., 1995. Induction of micronuclei by five pyrethroid insecticides in whole-blood and isolated human lymphocyte cultures. Mutation Research, 341, 169-184.
  • Taju, G., Abdul Majeed, S., Nambi, K.S.N., Farook, M.A., Vimal, S., Sahul Hameed, A.S., 2014. In vitro cytotoxic, genotoxic and oxidative stress of cypermethrin on five fish cell lines. Pesticide Biochemistry and Physiology, 113, 15-24.
  • Valles, S.M. Koehler, P.G., 2003. Insecticides used in the urban environment: mode of action (ENY282) Gainesville, FL: Department of Entomology and Nematology University of Florida.
  • Vardavas, A.I., Stivaktakis, P.D., Tzatzarakis, M.N., Fragkiadaki, P., Vasilaki, F., Tzardi, M., Datseri, G., Tsiaoussis, J., Alegakis, A.K., Tsitsimpikou, C., Rakitskii, V.N., Carvalho, V., Tsatsakis, A.M., 2016. Long-term exposure to cypermethrin and piperonyl butoxide cause liver and kidney inflammation and induce genotoxicity in New Zealand white male rabbits. Food and Chemical Toxicology, 94, 250-259.
  • Viel, J.F., Warembourg, C., Le Maner-Idrissi, G., Lacroix, A., Limon, G., Rouget, F., Monfort, C., Durand, G., Cordier, S., Chevrier, C., 2015. Pyrethroid insecticide exposure and cognitive developmental disabilities in children: the PELAGIE mother child cohort. Environment International, 82, 69-75.
  • Weisburger, J.H., 2001. Antimutagenesis and anticarcinogenesis, from the past to the future. Mutation Research, 480, 23-35.
  • Yun, X., Huang, Q., Rao, W., Xiao, C., Zhang, T., Mao, Z., Wan, Z.A., 2017. Comparative assessment of cytotoxicity of commonly used agricultural insecticides to human and insect cells. Ecotoxicology and Environmental Safety, 137, 179-185.

Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test

Year 2022, Volume: 22 Issue: 3, 465 - 469, 30.06.2022

Arzu Özkara

https://doi.org/10.35414/akufemubid.1069842

Abstract

Pesticides widely used in the agricultural market led to an increase in the quality and yield of crops. However, pesticides are a major concern in the environment as they also harm non-target creatures, because of pesticide resistance, and mutagenic and carcinogenic effects. In this study, we aimed to research the potential mutagenicity of Karate Zeon which is a synthetic pyrethroid insecticide. Salmonella typhimurium mutagenicity assay (Ames test) was used to evaluate the mutagenicity of Karate Zeon insecticide in the absence and presence of the S9 fraction on two strains (TA98 and TA100). In this study, doses of 250, 25, 2.5, 0.25, 0.025 μg/plate of Karate Zeon were used. Karate Zeon pesticide showed mutagenic activity at 250 μg/plate concentration on both TA98 and TA100 with and without metabolic activation.

Keywords

Ames test Karate zeon Mutagenicity Pesticide

References

  • Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological profile for pyrethrins and pyrethroids. Atlanta, GA: Department of Health and Human Services, Public Health Service, Toxicology (2003).
  • Ahouangninou, C., Martin, T., Edorh, P., Bio-Bangana, S., Samuel, O., St-Laurent, L. and Fayomi, B., 2012. Characterization of health and environmental risks of pesticide use in market-gardening in the rural city of Tori-Bossito in Benin, West Africa. Journal of Environmental Protection, 3, 241-248.
  • Ames, B.N., McCann, J., Yamasaki, E., 1975. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutation Research, 31, 347-364.
  • Asita, A.O. and Mokhobo, M.M., 2013. Clastogenic and cytotoxic effects of four pesticides used to control insect pests of stored products on root meristems of Allium cepa. Environment and Natural Resources Journal, 3(2), 133-145.
  • Basri, H., Topaktaş, M., Rencüzoğulları, E., Kayraldız, A., Dönbak, L., Dağlıoğlu, Y.K., 2008. Genotoxic potential of cyfluthrin. Mutation Research, 1(2), 49-50.
  • Casida, J., Quistad, G., 1998. Golden age of insecticide research: past, present, or future? Annual Review of Entomology, 43, 1-16.
  • Grossman, N., 2007. Influence of pyrethroids and piperonylbutoxide on histamine release from isolated rat mast cells. Journal of Inflammation Research, 56, 473-478.
  • Jensen, H.K., Konradsen, F., Dalsgaard, A., 2011. Pesticide use and self-reported symptoms of acute pesticide poisoning among aquatic farmers in Phnom Penh, Cambodia. International Journal of Toxicology, 639814.
  • Leme, D.M. and Marin-Morales, M.A., 2009. Allium cepa test in environmental monitoring: A review on its application. Mutation Research, 682, 71-81.
  • Maron, D.M., Ames, B.N., 1983. Revised methods for the Salmonella mutagenicity test. Mutation Research, 113, 173-215.
  • Medina, D., Prieto, A., Ettiene, G., Buscema, I. and Abreu de, V.A., 1999. Persistence of Organophosphorus Pesticide Residues in Limón River Waters. Bulletin of Environmental Contamination and Toxicology, 63(1), 39-44.
  • Miadokova, E., Vickova, V., Duhova, V., Trebaticka, M., Garajova, K., Grolmus, J., Podstavkova, S., Vicek, D., 1992. Effects of supercypermethrin, a synthetic developmental pyrethroid, on four biological test systems. Mutation Research, 3, 161-168.
  • Ministry of the Environment Compilation of pollutant release and transfer register data Ministry of the Environment; in Japanese, 2011.
  • Osman, K.A., Al-Humaid, A.M., Al-Rehiayani, S.M. and Al- Redhaiman, K.N., 2010. Monitoring of pesticide residues in vegetables marketed in Al-Qassim region, Saudi Arabia. Ecotoxicology and Environmental Safety, 73(6), 1433-1439.
  • Oulhote, Y., Bouchard, M.F., 2013. Urinary metabolites of organophosphate and pyrethroid pesticides and behavioral problems in Canadian children. Environmental Health Perspectives, 121, 1378-1384.
  • Özkara, A., 2017. Evaluation of Siperkor Pesticide Mutagenicity with Ames Test. Afyon Kocatepe University Journal of Sciences and Engineering, 17, 393-398.
  • Özkara, A., 2019. Assessment of cytotoxicity and mutagenicity of insecticide Demond EC25 in Allium cepa and Ames Test. Caryologia, 72(2), 21-27.
  • Pimentel, D., Greiner, A. and Bashore, T., 1998. Economic and environmental costs of pesticides use, Environmental Toxicology, Current Developments, London UK, Gordon and Breach Science Publisher, 121-187.
  • Pluijmen, M., Drevon, C., Montesano, R., Malaveille, C., Hautefeuille, A., Bartsch, H., 1984. Lack of mutagenicity of synthetic pyrethroids in Salmonella typhimurium strains and in V79, Chinese hamster ovary cells. Mutation Research, 137, 7-15.
  • Saillenfait, A.M., Ndiaye, D., Sabaté, J.P., 2015. Pyrethroids: exposure and health effects an update. International Journal of Hygiene and Environmental Health, 218(3), 281-92.
  • Shukla, Y., Taneja, P., 2000. Mutagenic evaluation of deltamethrin using rodent dominant lethal assay. Mutation Research, 467, 119-127.
  • Song, Y., Kai, J., Song, X., Zhang, W., Li, L., 2015. Long-term toxic effects of deltamethrin and fenvalerante in soil. Journal of Hazardous Materials, 289, 158-164.
  • Surralles, J., Xamena, N., Creus, A., Catalan, J., Norppa, H., Marcos, R., 1995. Induction of micronuclei by five pyrethroid insecticides in whole-blood and isolated human lymphocyte cultures. Mutation Research, 341, 169-184.
  • Taju, G., Abdul Majeed, S., Nambi, K.S.N., Farook, M.A., Vimal, S., Sahul Hameed, A.S., 2014. In vitro cytotoxic, genotoxic and oxidative stress of cypermethrin on five fish cell lines. Pesticide Biochemistry and Physiology, 113, 15-24.
  • Valles, S.M. Koehler, P.G., 2003. Insecticides used in the urban environment: mode of action (ENY282) Gainesville, FL: Department of Entomology and Nematology University of Florida.
  • Vardavas, A.I., Stivaktakis, P.D., Tzatzarakis, M.N., Fragkiadaki, P., Vasilaki, F., Tzardi, M., Datseri, G., Tsiaoussis, J., Alegakis, A.K., Tsitsimpikou, C., Rakitskii, V.N., Carvalho, V., Tsatsakis, A.M., 2016. Long-term exposure to cypermethrin and piperonyl butoxide cause liver and kidney inflammation and induce genotoxicity in New Zealand white male rabbits. Food and Chemical Toxicology, 94, 250-259.
  • Viel, J.F., Warembourg, C., Le Maner-Idrissi, G., Lacroix, A., Limon, G., Rouget, F., Monfort, C., Durand, G., Cordier, S., Chevrier, C., 2015. Pyrethroid insecticide exposure and cognitive developmental disabilities in children: the PELAGIE mother child cohort. Environment International, 82, 69-75.
  • Weisburger, J.H., 2001. Antimutagenesis and anticarcinogenesis, from the past to the future. Mutation Research, 480, 23-35.
  • Yun, X., Huang, Q., Rao, W., Xiao, C., Zhang, T., Mao, Z., Wan, Z.A., 2017. Comparative assessment of cytotoxicity of commonly used agricultural insecticides to human and insect cells. Ecotoxicology and Environmental Safety, 137, 179-185.
There are 29 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Arzu Özkara 0000-0002-7815-5366

Publication Date June 30, 2022
Submission Date February 8, 2022
Published in Issue Year 2022 Volume: 22 Issue: 3

Cite

APA Özkara, A. (2022). Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, 22(3), 465-469. https://doi.org/10.35414/akufemubid.1069842
AMA Özkara A. Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. June 2022;22(3):465-469. doi:10.35414/akufemubid.1069842
Chicago Özkara, Arzu. “Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 22, no. 3 (June 2022): 465-69. https://doi.org/10.35414/akufemubid.1069842.
EndNote Özkara A (June 1, 2022) Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 22 3 465–469.
IEEE A. Özkara, “Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test”, Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, vol. 22, no. 3, pp. 465–469, 2022, doi: 10.35414/akufemubid.1069842.
ISNAD Özkara, Arzu. “Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 22/3 (June 2022), 465-469. https://doi.org/10.35414/akufemubid.1069842.
JAMA Özkara A. Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. 2022;22:465–469.
MLA Özkara, Arzu. “Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, vol. 22, no. 3, 2022, pp. 465-9, doi:10.35414/akufemubid.1069842.
Vancouver Özkara A. Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. 2022;22(3):465-9.
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