Research Article
Neonikotinoid İnsektisitler İmidakloprid ve Tiyamethoksam’ın Ekmek Mayası (Saccharomyces cerevisiae) Glutatyon Redüktaz Enzim Aktivitesi Üzerine in vitro Etkisi
Abstract
Bu çalışmada, ekmek mayası (Saccharomyces cerevisiae) glutatyon redüktazı (GR), 0, 25, 50, 100, 250 ve 500 mg/L neonikotinoid insektisit imidakloprid (IMI) ve tiyamethoksam’a (TMX) maruz bırakılmıştır. 25, 50, 100, 250 ve 500 mg/L konsantrasyonlara maruz bırakılma ile, % GR aktivitesindeki değişimler, IMI uygulamalarında sırasıyla; -1.01; +0.50; 0.00; 0.00 ve -1.01 olarak hesaplanırken, TMX uygulamalarında sırasıyla -2.91; -1.46; -0.97; -1.46 ve -0.97 olarak hesaplanmıştır. Kontrol aktivitesine göre GR aktivitelerinde istatistiksel bir değişiklik gözlemlenmemiştir (p>0.05). Bu çalışma, test edilen neonikotinoid insektisitlerin GR enzim aktivitelerinde önemli değişikliklere neden olmadığını göstermiştir.
References
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- [5] Food and Agriculture Organization of the United Nations (FAO), FAO Specifıcations and Evaluations for Agricultural Pesticides: Thiamethoxam, 2014. Retrieved from: http://www.fao.org/fileadmin/templates/agphome/documents/Pests_ Pesticides / Specs/Thiamethoxam2014.pdf, 2018.
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- [9] Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the folin phenol reagent, Journal of Biological Chemistry, 193, 265–275, 1951.
- [10] Carlberg, I., Mannervik, B., Purification and characterization of the flavoenzyme glutathione reductase from rat liver, Journal of Biological Chemistry, 250(14), 5475–5480, 1975.
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- [13] Demirci, Ö., Güven, K., Asma, D., Öğüt, S., Uğurlu, P., Effects of endosulfan, thiamethoxam, and indoxacarb in combination with atrazine on multi-biomarkers in Gammarus kischineffensis, Ecotoxicology and Environmental Safety, 147, 749–758, 2018.
The in vitro Effect of Neonicotinoid Insecticides Imidacloprid and Thiamethoxam on Glutathione Reductase Enzyme Activity from Baker's Yeast (Saccharomyces cerevisiae)
Abstract
In this study, glutathione reductase from baker's yeast (Saccharomyces cerevisiae) (GR) exposed to 0, 25, 50, 100, 250 and 500 mg/L neonicotinoid insecticides imidacloprid (IMI) and thiamethoxam (TMX). Under the exposure of 25, 50, 100, 250 and 500 mg/L concentrations, % GR activity alterations were calculated as -1.01; +0.50; 0.00; 0.00 and -1.01 in IMI applications, while these alterations were calculated as -2.91; -1.46; -0.97; -1.46 and -0.97 in TMX applications, respectively. According to control activity, no statistical changes were observed in GR activities (p>0.05). The present study showed that the tested neonicotinoid insecticides did not cause significant changes in GR enzyme activities.
References
- [1] Halliwell, B., Gutteridge, J. M. C., Free radicals in biology and medicine, Third Edition, Oxford University Press., New York, 1999.
- [2] Thienne, R., Pai, E.F., Schirmer, R.H., and Schultz, G.E., Three-dimensional structure of glutathione reductase at 2å resolution, Journal of Molecular Biology, 152, 763–782, 1981.
- [3] Food and Agriculture Organization of the United Nations (FAO), FAO Specifıcations and Evaluations for Agricultural Pesticides: Imidacloprid, 2013. Retrieved from:http://www.fao.org/fileadmin/templates/agphome/documents/Pests_Pesticides/Specs/ Imidacloprid_2013.pdf, 2018.
- [4] Gervais, J.A., Luukinen, B., Buhl, K., Stone, D., Imidacloprid Technical Fact Sheet. National Pesticide Information Center, April, 2010.
- [5] Food and Agriculture Organization of the United Nations (FAO), FAO Specifıcations and Evaluations for Agricultural Pesticides: Thiamethoxam, 2014. Retrieved from: http://www.fao.org/fileadmin/templates/agphome/documents/Pests_ Pesticides / Specs/Thiamethoxam2014.pdf, 2018.
- [6] Rose, P. H., Selective toxicity of nicotine and neonicotinoids, In Marrs, Timothy C., Mammalian Toxicology of Insecticides, The Royal Society of Chemistry, 2012; 186 pp. [7] Carrington, D., Total ban on bee-harming pesticides likely after major new eu analysis, The Guardian, 28 February, 2018. Retrieved November, 2018.
- [8] Carrington, D., EU agrees total ban on bee-harming pesticides, The Guardian, 27 April, 2018. Retrieved November, 2018.
- [9] Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the folin phenol reagent, Journal of Biological Chemistry, 193, 265–275, 1951.
- [10] Carlberg, I., Mannervik, B., Purification and characterization of the flavoenzyme glutathione reductase from rat liver, Journal of Biological Chemistry, 250(14), 5475–5480, 1975.
- [11] Karadag, H., Bilgin, R., effect of cyprodinil and fludioxonil pesticides on human superoxide dismutase, Asian Journal of Chemistry, 22(10), 8147-8154, 2010.
- [12] Iturburu, F.G., Bertrand, L., Mendieta, J.R., Amé, M.V., Menone, M.L., An integrated biomarker response study explains more than the sum of the parts: oxidative stress in the fish Australoheros facetus exposed to imidacloprid, Ecological Indicators, 93, 351–357, 2018.
- [13] Demirci, Ö., Güven, K., Asma, D., Öğüt, S., Uğurlu, P., Effects of endosulfan, thiamethoxam, and indoxacarb in combination with atrazine on multi-biomarkers in Gammarus kischineffensis, Ecotoxicology and Environmental Safety, 147, 749–758, 2018.
There are 12 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Biology |
| Authors | |
| Publication Date | December 30, 2019 |
| Submission Date | March 1, 2019 |
| Acceptance Date | December 18, 2019 |
| Published in Issue | Year 2019 Volume: 9 Issue: 2 |
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